Bovine Ubiquitin-like protein ISG15(ISG15) ELISA kit CSB-EL011843BO CUSABIO
$50.00
In stock
SKU
CSB-EL011843BO
Product Details
| Target Name | ISG15 ubiquitin-like modifier |
| Uniprot No. | O02741 |
| Species | Bos taurus (Bovine) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.078 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | ISG15 ELISA Kit; G1P2 ELISA Kit; ISG17 ELISA Kit; UCRP ELISA Kit; Ubiquitin-like protein ISG15 ELISA Kit; Interferon-stimulated gene product 17 ELISA Kit; Ubiquitin cross-reactive protein ELISA Kit; BoUCRP ELISA Kit |
| Abbreviation | ISG15 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.312 ng/mL-20 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of bovine ISG15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of bovine ISG15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of bovine ISG15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 95; 1: Range %; 2: 85-102; 1: 1:2; 2: Average %; 3: 92; 1: Range %; 2: 83-99; 1: 1:4; 2: Average %; 3: 90; 1: Range %; 2: 85-97; 1: 1:8; 2: Average %; 3: 95; 1: Range %; 2: 83-103 |
| Recovery | headers: The recovery of bovine ISG15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of bovine ISG15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of bovine ISG15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 90-100; 1: EDTA plasma (n=4); 2: 93; 3: 86-105; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: ng/ml OD1 OD2 Average Corrected 20 2.105 1.995 2.050 1.871 10 1.654 1.627 1.641 1.462 5 1.153 1.185 1.169 0.990 2.5 0.772 0.757 0.765 0.586 1.25 0.537 0.534 0.536 0.357 0.625 0.364 0.355 0.360 0.181 0.312 0.262 0.275 0.269 0.090 0 0.177 0.181 0.179; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 9: 20; 10: 2.105; 11: 1.995; 12: 2.050; 13: 1.871; 16: 10; 17: 1.654; 18: 1.627; 19: 1.641; 20: 1.462; 23: 5; 24: 1.153; 25: 1.185; 26: 1.169; 27: 0.990; 30: 2.5; 31: 0.772; 32: 0.757; 33: 0.765; 34: 0.586; 37: 1.25; 38: 0.537; 39: 0.534; 40: 0.536; 41: 0.357; 44: 0.625; 45: 0.364; 46: 0.355; 47: 0.360; 48: 0.181; 51: 0.312; 52: 0.262; 53: 0.275; 54: 0.269; 55: 0.090; 58: 0; 59: 0.177; 60: 0.181; 61: 0.179; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 20; 2: 2.105; 3: 1.995; 4: 2.050; 5: 1.871; 1: 10; 2: 1.654; 3: 1.627; 4: 1.641; 5: 1.462; 1: 5; 2: 1.153; 3: 1.185; 4: 1.169; 5: 0.990; 1: 2.5; 2: 0.772; 3: 0.757; 4: 0.765; 5: 0.586; 1: 1.25; 2: 0.537; 3: 0.534; 4: 0.536; 5: 0.357; 1: 0.625; 2: 0.364; 3: 0.355; 4: 0.360; 5: 0.181; 1: 0.312; 2: 0.262; 3: 0.275; 4: 0.269; 5: 0.090; 1: 0; 2: 0.177; 3: 0.181; 4: 0.179 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Bovine ISG15 ELISA Kit was designed for the quantitative measurement of Bovine ISG15 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL. |
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