Human cAMP response element-binding protein (CREB) ELISA Kit CSB-E13527h CUSABIO
$50.00
In stock
SKU
CSB-E13527h
Product Details
| Target Name | cAMP responsive element binding protein 1 |
| Uniprot No. | P16220 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.078 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Active transcription factor CREB ELISA Kit; cAMP response element binding protein 1 ELISA Kit; cAMP response element binding protein ELISA Kit; cAMP responsive element binding protein 1 ELISA Kit; cAMP-responsive element-binding protein 1 ELISA Kit; CREB ELISA Kit; CREB-1 ELISA Kit; CREB1 ELISA Kit; CREB1_HUMAN ELISA Kit; Cyclic AMP-responsive element-binding protein 1 ELISA Kit; MGC9284 ELISA Kit; OTTHUMP00000163864 ELISA Kit; OTTHUMP00000163865 ELISA Kit; OTTHUMP00000206660 ELISA Kit; OTTHUMP00000206662 ELISA Kit; OTTHUMP00000206667 ELISA Kit; Transactivator protein ELISA Kit |
| Abbreviation | CREB1 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.312 ng/mL-20 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human CREB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human CREB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human CREB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:5; 2: Average %; 3: 92; 1: Range %; 2: 87-96; 1: 1:10; 2: Average %; 3: 89; 1: Range %; 2: 85-94; 1: 1:20; 2: Average %; 3: 95; 1: Range %; 2: 89-98; 1: 1:40; 2: Average %; 3: 102; 1: Range %; 2: 98-105 |
| Recovery | headers: The recovery of human CREB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human CREB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human CREB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 98; 3: 93-102; 1: EDTA plasma (n=4); 2: 86; 3: 80-92; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 20 2.157 2.254 2.206 2.104 10 1.715 1.812 1.764 1.662 5 1.267 1.284 1.276 1.174 2.5 0.857 0.863 0.860 0.758 1.25 0.523 0.532 0.528 0.426 0.625 0.343 0.351 0.347 0.245 0.312 0.206 0.213 0.210 0.108 0 0.103 0.101 0.102; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 20; 9: 2.157; 10: 2.254; 11: 2.206; 12: 2.104; 14: 10; 15: 1.715; 16: 1.812; 17: 1.764; 18: 1.662; 20: 5; 21: 1.267; 22: 1.284; 23: 1.276; 24: 1.174; 26: 2.5; 27: 0.857; 28: 0.863; 29: 0.860; 30: 0.758; 32: 1.25; 33: 0.523; 34: 0.532; 35: 0.528; 36: 0.426; 38: 0.625; 39: 0.343; 40: 0.351; 41: 0.347; 42: 0.245; 44: 0.312; 45: 0.206; 46: 0.213; 47: 0.210; 48: 0.108; 50: 0; 51: 0.103; 52: 0.101; 53: 0.102; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 20; 2: 2.157; 3: 2.254; 4: 2.206; 5: 2.104; 1: 10; 2: 1.715; 3: 1.812; 4: 1.764; 5: 1.662; 1: 5; 2: 1.267; 3: 1.284; 4: 1.276; 5: 1.174; 1: 2.5; 2: 0.857; 3: 0.863; 4: 0.860; 5: 0.758; 1: 1.25; 2: 0.523; 3: 0.532; 4: 0.528; 5: 0.426; 1: 0.625; 2: 0.343; 3: 0.351; 4: 0.347; 5: 0.245; 1: 0.312; 2: 0.206; 3: 0.213; 4: 0.210; 5: 0.108; 1: 0; 2: 0.103; 3: 0.101; 4: 0.102 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human CREB1 ELISA Kit was designed for the quantitative measurement of Human CREB1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL. |
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