Human cluster of differentiation 63,CD63 ELISA Kit CSB-E14107h CUSABIO
$50.00
In stock
SKU
CSB-E14107h
Product Details
| Target Name | CD63 molecule |
| Uniprot No. | P08962 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.156 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | CD63; MLA1; TSPAN30; CD63 antigen; Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Lysosome integral membrane protein 1; Limp1; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; Tspan-30; CD antigen CD63 |
| Abbreviation | CD63 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.625 ng/mL-40 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human CD63 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human CD63 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human CD63 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 96; 1: Range %; 2: 92-104; 1: 1:2; 2: Average %; 3: 87; 1: Range %; 2: 81-94; 1: 1:4; 2: Average %; 3: 98; 1: Range %; 2: 92-106; 1: 1:8; 2: Average %; 3: 96; 1: Range %; 2: 92-103 |
| Recovery | headers: The recovery of human CD63 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human CD63 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human CD63 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 104; 3: 98-107; 1: EDTA plasma (n=4); 2: 93; 3: 89-103; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 40 2.639 2.538 2.589 2.428 20 1.947 1.846 1.897 1.736 10 1.301 1.312 1.307 1.146 5 0.776 0.765 0.771 0.610 2.5 0.473 0.462 0.468 0.307 1.25 0.336 0.347 0.342 0.181 0.625 0.246 0.240 0.243 0.082 0 0.162 0.160 0.161; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 40; 9: 2.639; 10: 2.538; 11: 2.589; 12: 2.428; 14: 20; 15: 1.947; 16: 1.846; 17: 1.897; 18: 1.736; 20: 10; 21: 1.301; 22: 1.312; 23: 1.307; 24: 1.146; 26: 5; 27: 0.776; 28: 0.765; 29: 0.771; 30: 0.610; 32: 2.5; 33: 0.473; 34: 0.462; 35: 0.468; 36: 0.307; 38: 1.25; 39: 0.336; 40: 0.347; 41: 0.342; 42: 0.181; 44: 0.625; 45: 0.246; 46: 0.240; 47: 0.243; 48: 0.082; 50: 0; 51: 0.162; 52: 0.160; 53: 0.161; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 40; 2: 2.639; 3: 2.538; 4: 2.589; 5: 2.428; 1: 20; 2: 1.947; 3: 1.846; 4: 1.897; 5: 1.736; 1: 10; 2: 1.301; 3: 1.312; 4: 1.307; 5: 1.146; 1: 5; 2: 0.776; 3: 0.765; 4: 0.771; 5: 0.610; 1: 2.5; 2: 0.473; 3: 0.462; 4: 0.468; 5: 0.307; 1: 1.25; 2: 0.336; 3: 0.347; 4: 0.342; 5: 0.181; 1: 0.625; 2: 0.246; 3: 0.240; 4: 0.243; 5: 0.082; 1: 0; 2: 0.162; 3: 0.160; 4: 0.161 |
| Materials provided | A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human CD63 antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled CD63 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody. One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution. One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | CD63 is a transmembrane glycoprotein belonging to the tetraspanin family, characterized by four transmembrane domains and two extracellular loops. This molecule is predominantly localized in late endosomes and lysosomes, where it plays important roles in vesicular trafficking, membrane fusion, and cellular signaling processes. CD63 serves as a widely recognized marker for exosomes and is involved in various physiological functions including immune cell activation, platelet aggregation, and tumor progression. Its expression and secretion patterns make it a valuable biomarker for studying cellular communication and disease states. The Human cluster of differentiation 63,CD63 ELISA Kit (CSB-E14107h) uses a quantitative sandwich measurement approach to detect CD63 molecule in human samples. This assay accommodates serum, plasma, and tissue homogenates with a detection range of 0.625 ng/mL to 40 ng/mL and shows sensitivity of 0.156 ng/mL. The protocol requires 50-100ul sample volume and can be completed within 1-5 hours, with detection performed at 450 nm wavelength. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been used in extracellular vesicle research to quantify CD63 levels in small extracellular vesicles and evaluate preservation effects on vesicle contents. The kit serves as a tool for characterizing tetraspanin markers in isolated vesicles and measuring their protein composition across different experimental conditions. • Extracellular vesicle characterization - Quantification of CD63 tetraspanin levels in small extracellular vesicles to determine vesicle integrity and marker expression • Preservation studies - Evaluation of CD63 protein levels in extracellular vesicles following various storage and preservation protocols • Cell line-derived vesicle analysis - Detection and measurement of CD63 in extracellular vesicle preparations derived from different cellular sources • Stem cell research - Quantification of CD63 in lysed extracellular vesicles from human amniotic fluid-derived stem cells for vesicle characterization studies |
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