Human cytochrome c-1 (CYC1) ELISA kit CSB-E17854h CUSABIO
$50.00
In stock
SKU
CSB-E17854h
Product Details
| Target Name | cytochrome c-1 |
| Uniprot No. | P08574 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 7.8 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Complex III subunit 4 ELISA Kit; Complex III subunit IV ELISA Kit; CY1_HUMAN ELISA Kit; CYC1 ELISA Kit; Cytochrome b c1 complex subunit 4 ELISA Kit; Cytochrome b-c1 complex subunit 4 ELISA Kit; Cytochrome bc1 complex subunit 4 ELISA Kit; Cytochrome c 1 ELISA Kit; Cytochrome c-1 ELISA Kit; Cytochrome c1; heme protein; mitochondrial ELISA Kit; Ubiquinol cytochrome c reductase complex cytochrome c1 subunit ELISA Kit; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit ELISA Kit; UQCR4 ELISA Kit |
| Abbreviation | CYC1 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 31.25 pg/mL-2000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of Human CYC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of Human CYC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of Human CYC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 89; 1: Range %; 2: 85-93; 1: 1:2; 2: Average %; 3: 94; 1: Range %; 2: 90-98; 1: 1:4; 2: Average %; 3: 92; 1: Range %; 2: 88-96; 1: 1:8; 2: Average %; 3: 97; 1: Range %; 2: 93-101 |
| Recovery | headers: The recovery of Human CYC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of Human CYC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of Human CYC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 102; 3: 97-106; 1: EDTA plasma (n=4); 2: 87; 3: 83-91; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 2000 2.863 2.967 2.915 2.817 1000 2.526 2.425 2.476 2.378 500 1.875 1.776 1.826 1.728 250 1.065 1.043 1.054 0.956 125 0.547 0.524 0.536 0.438 62.5 0.284 0.272 0.278 0.180 31.25 0.198 0.189 0.194 0.096 0 0.097 0.098 0.098; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 2000; 9: 2.863; 10: 2.967; 11: 2.915; 12: 2.817; 14: 1000; 15: 2.526; 16: 2.425; 17: 2.476; 18: 2.378; 20: 500; 21: 1.875; 22: 1.776; 23: 1.826; 24: 1.728; 26: 250; 27: 1.065; 28: 1.043; 29: 1.054; 30: 0.956; 32: 125; 33: 0.547; 34: 0.524; 35: 0.536; 36: 0.438; 38: 62.5; 39: 0.284; 40: 0.272; 41: 0.278; 42: 0.180; 44: 31.25; 45: 0.198; 46: 0.189; 47: 0.194; 48: 0.096; 50: 0; 51: 0.097; 52: 0.098; 53: 0.098; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 2000; 2: 2.863; 3: 2.967; 4: 2.915; 5: 2.817; 1: 1000; 2: 2.526; 3: 2.425; 4: 2.476; 5: 2.378; 1: 500; 2: 1.875; 3: 1.776; 4: 1.826; 5: 1.728; 1: 250; 2: 1.065; 3: 1.043; 4: 1.054; 5: 0.956; 1: 125; 2: 0.547; 3: 0.524; 4: 0.536; 5: 0.438; 1: 62.5; 2: 0.284; 3: 0.272; 4: 0.278; 5: 0.180; 1: 31.25; 2: 0.198; 3: 0.189; 4: 0.194; 5: 0.096; 1: 0; 2: 0.097; 3: 0.098; 4: 0.098 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human CYC1 ELISA Kit was designed for the quantitative measurement of Human CYC1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL. |
MDSD