Human heat shock protein 90α (HSP-90α) ELISA kit CSB-E13462h CUSABIO
$50.00
In stock
SKU
CSB-E13462h
Product Details
| Target Name | heat shock protein 90α (HSP-90α) |
| Uniprot No. | P07900 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.195 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Heat shock 86 kDa ELISA Kit; Heat shock protein 90kDa alpha cytosolic class A member 1 ELISA Kit; Heat shock protein 90kDa alpha cytosolic class B member 1 ELISA Kit; Heat shock protein HSP 90 alpha ELISA Kit; Heat shock protein HSP 90 beta ELISA Kit; Heat shock protein HSP 90-alpha ELISA Kit; HS90A_HUMAN ELISA Kit; HSP 84 ELISA Kit; HSP 86 ELISA Kit; Hsp 90 ELISA Kit; HSP86 ELISA Kit; HSP90A ELISA Kit; HSP90AA1 ELISA Kit; HSP90AB1 ELISA Kit; HSP90B ELISA Kit; HSPC1 ELISA Kit; HSPC2 ELISA Kit; HSPCAL1 ELISA Kit; HSPCAL4 ELISA Kit; Renal carcinoma antigen NY-REN-38 ELISA Kit |
| Abbreviation | HSP90AA1 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.78 ng/mL-50 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Neuroscience |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human HSP-90α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human HSP-90α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human HSP-90α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:5; 2: Average %; 3: 93; 1: Range %; 2: 90-95; 1: 1:10; 2: Average %; 3: 104; 1: Range %; 2: 100-107; 1: 1:20; 2: Average %; 3: 94; 1: Range %; 2: 89-97; 1: 1:40; 2: Average %; 3: 99; 1: Range %; 2: 92-103 |
| Recovery | headers: The recovery of human HSP-90α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human HSP-90α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human HSP-90α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 94; 3: 89-97; 1: EDTA plasma (n=4); 2: 94; 3: 91-97; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 50 2.712 2.699 2.706 2.569 25 2.013 2.002 2.008 1.871 12.5 1.400 1.422 1.411 1.274 6.25 0.804 0.788 0.796 0.659 3.12 0.424 0.428 0.426 0.289 1.56 0.349 0.341 0.345 0.208 0.78 0.245 0.234 0.240 0.103 0 0.139 0.135 0.137; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 50; 9: 2.712; 10: 2.699; 11: 2.706; 12: 2.569; 14: 25; 15: 2.013; 16: 2.002; 17: 2.008; 18: 1.871; 20: 12.5; 21: 1.400; 22: 1.422; 23: 1.411; 24: 1.274; 26: 6.25; 27: 0.804; 28: 0.788; 29: 0.796; 30: 0.659; 32: 3.12; 33: 0.424; 34: 0.428; 35: 0.426; 36: 0.289; 38: 1.56; 39: 0.349; 40: 0.341; 41: 0.345; 42: 0.208; 44: 0.78; 45: 0.245; 46: 0.234; 47: 0.240; 48: 0.103; 50: 0; 51: 0.139; 52: 0.135; 53: 0.137; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 50; 2: 2.712; 3: 2.699; 4: 2.706; 5: 2.569; 1: 25; 2: 2.013; 3: 2.002; 4: 2.008; 5: 1.871; 1: 12.5; 2: 1.400; 3: 1.422; 4: 1.411; 5: 1.274; 1: 6.25; 2: 0.804; 3: 0.788; 4: 0.796; 5: 0.659; 1: 3.12; 2: 0.424; 3: 0.428; 4: 0.426; 5: 0.289; 1: 1.56; 2: 0.349; 3: 0.341; 4: 0.345; 5: 0.208; 1: 0.78; 2: 0.245; 3: 0.234; 4: 0.240; 5: 0.103; 1: 0; 2: 0.139; 3: 0.135; 4: 0.137 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Heat shock protein 90α (HSP-90α) is a molecular chaperone that plays a key role in protein folding, cellular signaling, and stress response mechanisms. As a member of the HSP90 family, this protein helps fold and stabilize many client proteins, including kinases, transcription factors, and steroid hormone receptors. Cells express HSP-90α constitutively, but levels increase under stress conditions. This makes the protein essential for cellular homeostasis and survival. Its role in cancer progression, neurodegenerative diseases, and inflammatory responses has made it an important target for biomedical research. The Human heat shock protein 90α (HSP-90α) ELISA kit (CSB-E13462h) provides quantitative detection of HSP-90α in human samples. This sandwich-based assay works with serum, plasma, and tissue homogenates. The detection range spans 0.78 ng/mL to 50 ng/mL with sensitivity of 0.195 ng/mL. The assay needs 50-100 μL sample volume and takes 1-5 hours to complete. Detection occurs at 450 nm wavelength, giving researchers a reliable tool for measuring HSP-90α levels in various biological specimens. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been used in research studies to quantify heat shock protein levels in human serum samples and cell culture media. The assay allows researchers to study protein expression changes across different experimental conditions and examine how cytotoxic substances affect heat shock protein expression. • Biomarker profiling - Quantification of heat shock protein levels in serum samples as part of multi-protein expression analysis comparing different experimental groups • Cell culture studies - Detection of secreted heat shock protein levels in cell culture supernatants to examine cellular protein secretion patterns • Cytotoxicity research - Evaluation of heat shock protein expression changes in response to cytotoxic substance exposure • Comparative expression analysis - Measurement of protein level changes between control and experimental conditions in research settings |
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