Bovine interleukin 4,IL-4 ELISA Kit CSB-E12898B CUSABIO
$50.00
In stock
SKU
CSB-E12898B
Product Details
| Target Name | interleukin 4 |
| Uniprot No. | P30367 |
| Species | Bos taurus (Bovine) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3.12 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit |
| Abbreviation | IL4 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 12.5 pg/mL-800 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 95; 1: Range %; 2: 89-99; 1: 1:2; 2: Average %; 3: 97; 1: Range %; 2: 92-103; 1: 1:4; 2: Average %; 3: 90; 1: Range %; 2: 85-94; 1: 1:8; 2: Average %; 3: 84; 1: Range %; 2: 80-88 |
| Recovery | headers: The recovery of bovine IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of bovine IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of bovine IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 89-99; 1: EDTA plasma (n=4); 2: 89; 3: 84-93; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 800 1.993 1.912 1.953 1.835 400 1.576 1.616 1.596 1.478 200 1.304 1.354 1.329 1.211 100 0.939 0.962 0.951 0.833 50 0.624 0.641 0.633 0.515 25 0.441 0.419 0.430 0.312 12.5 0.283 0.279 0.281 0.163 0 0.119 0.117 0.118; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 800; 9: 1.993; 10: 1.912; 11: 1.953; 12: 1.835; 14: 400; 15: 1.576; 16: 1.616; 17: 1.596; 18: 1.478; 20: 200; 21: 1.304; 22: 1.354; 23: 1.329; 24: 1.211; 26: 100; 27: 0.939; 28: 0.962; 29: 0.951; 30: 0.833; 32: 50; 33: 0.624; 34: 0.641; 35: 0.633; 36: 0.515; 38: 25; 39: 0.441; 40: 0.419; 41: 0.430; 42: 0.312; 44: 12.5; 45: 0.283; 46: 0.279; 47: 0.281; 48: 0.163; 50: 0; 51: 0.119; 52: 0.117; 53: 0.118; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 800; 2: 1.993; 3: 1.912; 4: 1.953; 5: 1.835; 1: 400; 2: 1.576; 3: 1.616; 4: 1.596; 5: 1.478; 1: 200; 2: 1.304; 3: 1.354; 4: 1.329; 5: 1.211; 1: 100; 2: 0.939; 3: 0.962; 4: 0.951; 5: 0.833; 1: 50; 2: 0.624; 3: 0.641; 4: 0.633; 5: 0.515; 1: 25; 2: 0.441; 3: 0.419; 4: 0.430; 5: 0.312; 1: 12.5; 2: 0.283; 3: 0.279; 4: 0.281; 5: 0.163; 1: 0; 2: 0.119; 3: 0.117; 4: 0.118 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Bovine IL4 ELISA Kit was designed for the quantitative measurement of Bovine IL4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL . |
MDSD
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