Human insulin-like growth factors 2,IGF-2 ELISA Kit CSB-E04583h CUSABIO
$50.00
In stock
SKU
CSB-E04583h
Product Details
| Target Name | insulin-like growth factor 2 (somatomedin A) |
| Uniprot No. | P01344 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 15.6 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | C11orf43 ELISA Kit; IGF 2 ELISA Kit; IGF II ELISA Kit; IGF-II ELISA Kit; IGF2 ELISA Kit; IGF2_HUMAN ELISA Kit; IGFII ELISA Kit; INSIGF ELISA Kit; insulin like growth factor 2 (somatomedin A) ELISA Kit; Insulin like Growth Factor 2 ELISA Kit; Insulin like growth factor II ELISA Kit; Insulin like growth factor II precursor ELISA Kit; Insulin like growth factor type 2 ELISA Kit; pp9974 ELISA Kit; Preptin ELISA Kit; putative insulin like growth factor II associated protein ELISA Kit; Somatomedin A ELISA Kit; Somatomedin-A ELISA Kit |
| Abbreviation | IGF2 |
| Sample Types | serum, plasma, cell culture supernates, urine, tissue homogenates |
| Detection Range | 62.5 pg/mL-4000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Metabolism |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human IGF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human IGF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human IGF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:200; 2: Average %; 3: 84; 1: Range %; 2: 80-92; 1: 1:400; 2: Average %; 3: 97; 1: Range %; 2: 91-105; 1: 1:800; 2: Average %; 3: 97; 1: Range %; 2: 92-104; 1: 1:1600; 2: Average %; 3: 93; 1: Range %; 2: 86-98 |
| Recovery | headers: The recovery of human IGF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human IGF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human IGF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 89-104; 1: EDTA plasma (n=4); 2: 97; 3: 90-106; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 4000 2.789 2.769 2.779 2.673 2000 2.034 2.046 2.040 1.934 1000 1.518 1.478 1.498 1.392 500 1.205 1.206 1.206 1.100 250 0.823 0.839 0.831 0.725 125 0.536 0.546 0.541 0.435 62.5 0.349 0.369 0.359 0.253 0 0.102 0.110 0.106; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 4000; 9: 2.789; 10: 2.769; 11: 2.779; 12: 2.673; 14: 2000; 15: 2.034; 16: 2.046; 17: 2.040; 18: 1.934; 20: 1000; 21: 1.518; 22: 1.478; 23: 1.498; 24: 1.392; 26: 500; 27: 1.205; 28: 1.206; 29: 1.206; 30: 1.100; 32: 250; 33: 0.823; 34: 0.839; 35: 0.831; 36: 0.725; 38: 125; 39: 0.536; 40: 0.546; 41: 0.541; 42: 0.435; 44: 62.5; 45: 0.349; 46: 0.369; 47: 0.359; 48: 0.253; 50: 0; 51: 0.102; 52: 0.110; 53: 0.106; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 4000; 2: 2.789; 3: 2.769; 4: 2.779; 5: 2.673; 1: 2000; 2: 2.034; 3: 2.046; 4: 2.040; 5: 1.934; 1: 1000; 2: 1.518; 3: 1.478; 4: 1.498; 5: 1.392; 1: 500; 2: 1.205; 3: 1.206; 4: 1.206; 5: 1.100; 1: 250; 2: 0.823; 3: 0.839; 4: 0.831; 5: 0.725; 1: 125; 2: 0.536; 3: 0.546; 4: 0.541; 5: 0.435; 1: 62.5; 2: 0.349; 3: 0.369; 4: 0.359; 5: 0.253; 1: 0; 2: 0.102; 3: 0.110; 4: 0.106 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | CUSABIO's IGF2 human ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGF2 in multiple samples, including serum, plasma, cell culture supernates, urine, and tissue homogenates. This assay uses an antibody specific to human IGF2 coated on a 96-well plate. IGF2 present in a sample is bound to the wells by the immobilized antibody after adding the sample to the wells. Biotinylated IGF2 antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF2 bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader. IGF2 is a protein hormone that regulates cell proliferation, growth, migration, differentiation, and survival. IGF2 binds to IGF2R and is targeted for degradation, while engagement of IGF2 to IGF1R stimulates signaling pathways involved in metabolism, proliferation, differentiation, and anti-apoptosis. It also plays an essential role in bone development, where it promotes the proliferation and differentiation of bone cells. IGF2 has been proposed to participate in the development of various seemingly unrelated cancers that play an important role in geriatric medicine, such as breast cancer, colon cancer, and lung cancer. IGF2 has also been involved in cardiovascular diseases. |
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