Human leukocyte antigen G,HLA-G ELISA Kit CSB-E09401h CUSABIO
$50.00
In stock
SKU
CSB-E09401h
Product Details
| Target Name | major histocompatibility complex, class I, G |
| Uniprot No. | P17693 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.31 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | B2 microglobulin ELISA Kit; DADB-15K14.8 ELISA Kit; HLA 6.0 ELISA Kit; HLA class I histocompatibility antigen alpha chain G ELISA Kit; HLA class I histocompatibility antigen; alpha chain G ELISA Kit; HLA class I molecule ELISA Kit; HLA G ELISA Kit; HLA G antigen ELISA Kit; HLA G histocompatibility antigen class I G ELISA Kit; HLA G3 ELISA Kit; HLA-G ELISA Kit; HLA-G histocompatibility antigen; class I ELISA Kit; HLA60 ELISA Kit; HLAG ELISA Kit; HLAG_HUMAN ELISA Kit; Major histocompatibility complex class I G ELISA Kit; MHC class I antigen ELISA Kit; MHC class I antigen G ELISA Kit; MHC G ELISA Kit; T-cell A locus ELISA Kit; TCA ELISA Kit |
| Abbreviation | HLA-G |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 1.25 ng/mL-80 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human HLA-G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human HLA-G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human HLA-G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 93; 1: Range %; 2: 86-98; 1: 1:2; 2: Average %; 3: 103; 1: Range %; 2: 97-108; 1: 1:4; 2: Average %; 3: 93; 1: Range %; 2: 85-98; 1: 1:8; 2: Average %; 3: 97; 1: Range %; 2: 91-101 |
| Recovery | headers: The recovery of human HLA-G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human HLA-G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human HLA-G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 86; 3: 80-94; 1: EDTA plasma (n=4); 2: 96; 3: 92-104; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 80 2.961 2.960 2.961 2.828 40 2.252 2.205 2.229 2.096 20 1.266 1.286 1.276 1.143 10 0.731 0.755 0.743 0.610 5 0.368 0.400 0.384 0.251 2.5 0.302 0.322 0.312 0.179 1.25 0.227 0.215 0.221 0.088 0 0.134 0.132 0.133; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 80; 9: 2.961; 10: 2.960; 11: 2.961; 12: 2.828; 14: 40; 15: 2.252; 16: 2.205; 17: 2.229; 18: 2.096; 20: 20; 21: 1.266; 22: 1.286; 23: 1.276; 24: 1.143; 26: 10; 27: 0.731; 28: 0.755; 29: 0.743; 30: 0.610; 32: 5; 33: 0.368; 34: 0.400; 35: 0.384; 36: 0.251; 38: 2.5; 39: 0.302; 40: 0.322; 41: 0.312; 42: 0.179; 44: 1.25; 45: 0.227; 46: 0.215; 47: 0.221; 48: 0.088; 50: 0; 51: 0.134; 52: 0.132; 53: 0.133; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 80; 2: 2.961; 3: 2.960; 4: 2.961; 5: 2.828; 1: 40; 2: 2.252; 3: 2.205; 4: 2.229; 5: 2.096; 1: 20; 2: 1.266; 3: 1.286; 4: 1.276; 5: 1.143; 1: 10; 2: 0.731; 3: 0.755; 4: 0.743; 5: 0.610; 1: 5; 2: 0.368; 3: 0.400; 4: 0.384; 5: 0.251; 1: 2.5; 2: 0.302; 3: 0.322; 4: 0.312; 5: 0.179; 1: 1.25; 2: 0.227; 3: 0.215; 4: 0.221; 5: 0.088; 1: 0; 2: 0.134; 3: 0.132; 4: 0.133 |
| Materials provided | A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human HLA-G antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled HLA-G antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody. One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution. One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human HLA-G ELISA Kit was designed for the quantitative measurement of Human HLA-G protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.31 ng/mL . |
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