Human melanin-concentrating hormone (PMCH) ELISA kit CSB-EL018230HU CUSABIO
$50.00
In stock
SKU
CSB-EL018230HU
Product Details
| Target Name | melanin-concentrating hormone (PMCH) |
| Uniprot No. | P20382 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3.9 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | PMCH ELISA Kit; MCHPro-MCH [Cleaved into: Neuropeptide-glycine-glutamic acid ELISA Kit; NGE ELISA Kit; Neuropeptide G-E); Neuropeptide-glutamic acid-isoleucine ELISA Kit; NEI ELISA Kit; Neuropeptide E-I); Melanin-concentrating hormone ELISA Kit; MCH)] ELISA Kit |
| Abbreviation | PMCH |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 15.6 pg/mL-1000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Neuroscience |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human PMCH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human PMCH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human PMCH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 95; 1: Range %; 2: 90-100; 1: 1:2; 2: Average %; 3: 87; 1: Range %; 2: 83-91; 1: 1:4; 2: Average %; 3: 92; 1: Range %; 2: 88-96; 1: 1:8; 2: Average %; 3: 90; 1: Range %; 2: 85-94 |
| Recovery | headers: The recovery of human PMCH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human PMCH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human PMCH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 100; 3: 95-104; 1: EDTA plasma (n=4); 2: 84; 3: 80-88; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml OD1 OD2 Average Corrected 1000 2.682 2.533 2.608 2.400 500 2.040 1.946 1.993 1.785 250 1.419 1.357 1.388 1.180 125 0.927 0.975 0.951 0.743 62.5 0.654 0.689 0.672 0.464 31.2 0.408 0.392 0.400 0.192 15.6 0.335 0.324 0.330 0.122 0 0.204 0.211 0.208; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 9: 1000; 10: 2.682; 11: 2.533; 12: 2.608; 13: 2.400; 16: 500; 17: 2.040; 18: 1.946; 19: 1.993; 20: 1.785; 23: 250; 24: 1.419; 25: 1.357; 26: 1.388; 27: 1.180; 30: 125; 31: 0.927; 32: 0.975; 33: 0.951; 34: 0.743; 37: 62.5; 38: 0.654; 39: 0.689; 40: 0.672; 41: 0.464; 44: 31.2; 45: 0.408; 46: 0.392; 47: 0.400; 48: 0.192; 51: 15.6; 52: 0.335; 53: 0.324; 54: 0.330; 55: 0.122; 58: 0; 59: 0.204; 60: 0.211; 61: 0.208; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 1000; 2: 2.682; 3: 2.533; 4: 2.608; 5: 2.400; 1: 500; 2: 2.040; 3: 1.946; 4: 1.993; 5: 1.785; 1: 250; 2: 1.419; 3: 1.357; 4: 1.388; 5: 1.180; 1: 125; 2: 0.927; 3: 0.975; 4: 0.951; 5: 0.743; 1: 62.5; 2: 0.654; 3: 0.689; 4: 0.672; 5: 0.464; 1: 31.2; 2: 0.408; 3: 0.392; 4: 0.400; 5: 0.192; 1: 15.6; 2: 0.335; 3: 0.324; 4: 0.330; 5: 0.122; 1: 0; 2: 0.204; 3: 0.211; 4: 0.208 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human PMCH ELISA Kit was designed for the quantitative measurement of Human PMCH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL. |
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