Recombinant Human C-C motif chemokine 7 protein (CCL7) (Active) CSB-AP000881HU CUSABIO
$354.00
In stock
SKU
CSB-AP000881HU
Product Details
| Uniprot No. | P80098 |
| Alternative Names | C-C motif chemokine 7; Ccl7; CCL7_HUMAN; Chemokine CC motif ligand 7; FIC; MARC; MCP-3; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; NC28; RP23-350G1.4; SCYA6; SCYA7; Small-inducible cytokine A7 |
| Species | Homo sapiens (Human) |
| Source | E.coli |
| Expression Region | 24-99aa |
| Purity | >97% as determined by SDS-PAGE. |
| Endotoxin | Less than 1.0 EU/μg as determined by LAL method. |
| Activity | Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml. |
| Tag Info | Tag-Free |
| Form | Lyophilized powder |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM PB, pH 7.4, 150 mM NaCl |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs | Protein FAQs |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Lead Time | 5-10 business days |
| Target Names | CCL7 |
| Research Area | Immunology |
| Complete Sequence | QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL |
| Mol. Weight | 9.0 kDa |
| Protein Length | Full Length of Mature Protein |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Datasheet & COA | Please contact us to get it. |
| Description | CCL7 (MCP-3) is a potent CC chemokine that recruits monocytes, dendritic cells, and lymphocytes through CCR1, CCR2, and CCR3 signaling, making it a critical mediator in inflammatory cell trafficking. This tag-free recombinant human CCL7, encompassing the full mature sequence (residues 24–99), demonstrates confirmed chemotactic activity on primary human monocytes at 10–100 ng/mL, providing a suitable basis for Transwell migration assays, monocyte activation studies, and downstream investigation of MAPK and NF-κB signaling pathways. The endotoxin level of less than 1.0 EU/μg minimizes LPS-driven artifacts that can confound immune cell functional readouts, supporting use in sensitive in vivo inflammation models and primary cell-based experiments where cytokine-specific effects must be distinguished from endotoxin contamination. Purity exceeding 97% by SDS-PAGE, combined with this stringent endotoxin profile, also satisfies the criteria typical for ELISA reference standards, antibody validation controls, and immunogen preparation. |
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