Human sex hormone-binding globulin,SHBG ELISA Kit CSB-E08232h CUSABIO
$50.00
In stock
SKU
CSB-E08232h
Product Details
| Target Name | sex hormone-binding globulin |
| Uniprot No. | P04278 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.39 pmol/L |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | ABP ELISA Kit; Androgen binding protein ELISA Kit; SBP ELISA Kit; Sex hormone-binding globulin ELISA Kit; Sex steroid binding protein ELISA Kit; Sex steroid-binding protein ELISA Kit; SHBG ELISA Kit; SHBG_HUMAN ELISA Kit; TeBG ELISA Kit; Testis specific androgen binding protein ELISA Kit; Testis-specific androgen-binding protein ELISA Kit; Testosterone binding beta globulin ELISA Kit; Testosterone estradiol binding globulin ELISA Kit; Testosterone estrogen binding globulin ELISA Kit; Testosterone-estradiol-binding globulin ELISA Kit; Testosterone-estrogen-binding globulin ELISA Kit |
| Abbreviation | SHBG |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 1.56 pmol/L- 100 pmol/L |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; 1: Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean(pmol/L) 12.024 12.172 12.197 12.049 12.147 12.271 SD 0.039 0.049 0.058 0.048 0.059 0.065 CV(%) 4.442 5.543 6.554 5.461 6.682 7.320; 3: Intra-Assay Precision; 4: Inter-Assay Precision; 5: Sample; 6: 1; 7: 2; 8: 3; 9: 1; 10: 2; 11: 3; 12: n; 13: 20; 14: 20; 15: 20; 16: 20; 17: 20; 18: 20; 19: Mean(pmol/L); 20: 12.024; 21: 12.172; 22: 12.197; 23: 12.049; 24: 12.147; 25: 12.271; 26: SD; 27: 0.039; 28: 0.049; 29: 0.058; 30: 0.048; 31: 0.059; 32: 0.065; 33: CV(%); 34: 4.442; 35: 5.543; 36: 6.554; 37: 5.461; 38: 6.682; 39: 7.320; 2: Intra-Assay Precision; 3: Inter-Assay Precision; 1: Sample; 2: 1; 3: 2; 4: 3; 5: 1; 6: 2; 7: 3; 1: n; 2: 20; 3: 20; 4: 20; 5: 20; 6: 20; 7: 20; 1: Mean(pmol/L); 2: 12.024; 3: 12.172; 4: 12.197; 5: 12.049; 6: 12.147; 7: 12.271; 1: SD; 2: 0.039; 3: 0.049; 4: 0.058; 5: 0.048; 6: 0.059; 7: 0.065; 1: CV(%); 2: 4.442; 3: 5.543; 4: 6.554; 5: 5.461; 6: 6.682; 7: 7.320 |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1000; 2: Average %; 3: 96; 1: Range %; 2: 91-100; 1: 1:2000; 2: Average %; 3: 92; 1: Range %; 2: 88-97; 1: 1:4000; 2: Average %; 3: 99; 1: Range %; 2: 94-103; 1: 1:8000; 2: Average %; 3: 84; 1: Range %; 2: 80-88; |
| Recovery | headers: The recovery of human SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 94; 3: 89-98; 1: EDTA plasma (n=4); 2: 90; 3: 85-94; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; ; ; ; ; ; ; ; ; ; ; 1: pmol/L; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 100; 2: 2.132; 3: 2.092; 4: 2.112; 5: 1.912; 1: 50; 2: 1.701; 3: 1.699; 4: 1.700; 5: 1.519; 1: 25; 2: 1.298; 3: 1.304; 4: 1.301; 5: 1.124; 1: 12.5; 2: 0.845; 3: 0.850; 4: 0.848; 5: 0.670; 1: 6.25; 2: 0.628; 3: 0.633; 4: 0.631; 5: 0.453; 1: 3.12; 2: 0.388; 3: 0.395; 4: 0.391; 5: 0.215; 1: 1.56; 2: 0.279; 3: 0.274; 4: 0.276; 5: 0.094; 1: 0; 2: 0.178; 3: 0.181; 4: 0.180; |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Sex hormone-binding globulin (SHBG) is a glycoprotein produced primarily in the liver that serves as the major transport protein for sex hormones including testosterone, dihydrotestosterone, and estradiol in blood circulation. SHBG regulates the bioavailability of these hormones by binding them with high affinity, thereby controlling the amount of free, biologically active hormones available to target tissues. Changes in SHBG levels are associated with various physiological and pathological conditions, making it an important biomarker in endocrinology research and clinical diagnostics related to reproductive health, metabolic disorders, and hormonal imbalances. The Human sex hormone-binding globulin SHBG ELISA Kit (CSB-E08232h) provides quantitative measurement of SHBG in human samples. This sandwich ELISA works with serum, plasma, and tissue homogenates. Detection range spans 1.56 pmol/L to 100 pmol/L with sensitivity of 0.39 pmol/L. The assay requires 50-100 μL sample volume and takes 1-5 hours to complete, with detection performed at 450 nm wavelength. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. Researchers have used this ELISA kit to study sex hormone-binding globulin levels across diverse physiological and pathological contexts. Studies have measured SHBG concentrations in serum samples as part of comprehensive biochemical profiling and endocrine evaluations. • Sexual function research: Measuring SHBG alongside other sex hormones and enzymes in tissue homogenates to evaluate reproductive and sexual health parameters • Cardiovascular and metabolic studies: Determining serum SHBG concentrations in conjunction with lipid profiles and inflammatory markers to explore relationships between sex hormone-binding proteins and metabolic health • Endocrine profiling: Quantifying SHBG as part of broader hormonal panels to characterize endocrine status and hormone-binding protein dynamics |
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