Human soluble platelet endothelial cell adhesion molecule 1,sPECAM-1 ELISA Kit CSB-E08133h CUSABIO
$50.00
In stock
SKU
CSB-E08133h
Product Details
| Target Name | soluble platelet endothelial cell adhesion molecule 1,sPECAM-1 |
| Uniprot No. | P16284 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 7.8 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | PECAM1; Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31 |
| Abbreviation | sPECAM1/sCD31 |
| Sample Types | serum, plasma, cell culture supernates |
| Detection Range | 31.25 pg/mL-2000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Others |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human SPECAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human SPECAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human SPECAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:100; 2: Average %; 3: 95; 1: Range %; 2: 90-100; 1: 1:200; 2: Average %; 3: 88; 1: Range %; 2: 84-93; 1: 1:400; 2: Average %; 3: 100; 1: Range %; 2: 95-110; 1: 1:800; 2: Average %; 3: 90; 1: Range %; 2: 85-97 |
| Recovery | headers: The recovery of human SPECAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human SPECAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human SPECAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 84; 3: 80-90; 1: EDTA plasma (n=4); 2: 93; 3: 89-98; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 2000 2.468 2.324 2.396 2.302 1000 1.931 1.852 1.892 1.798 500 1.224 1.176 1.200 1.106 250 0.568 0.602 0.585 0.491 125 0.333 0.341 0.337 0.243 62.5 0.237 0.230 0.234 0.140 31.25 0.166 0.162 0.164 0.070 0 0.093 0.094 0.094; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 2000; 9: 2.468; 10: 2.324; 11: 2.396; 12: 2.302; 14: 1000; 15: 1.931; 16: 1.852; 17: 1.892; 18: 1.798; 20: 500; 21: 1.224; 22: 1.176; 23: 1.200; 24: 1.106; 26: 250; 27: 0.568; 28: 0.602; 29: 0.585; 30: 0.491; 32: 125; 33: 0.333; 34: 0.341; 35: 0.337; 36: 0.243; 38: 62.5; 39: 0.237; 40: 0.230; 41: 0.234; 42: 0.140; 44: 31.25; 45: 0.166; 46: 0.162; 47: 0.164; 48: 0.070; 50: 0; 51: 0.093; 52: 0.094; 53: 0.094; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 2000; 2: 2.468; 3: 2.324; 4: 2.396; 5: 2.302; 1: 1000; 2: 1.931; 3: 1.852; 4: 1.892; 5: 1.798; 1: 500; 2: 1.224; 3: 1.176; 4: 1.200; 5: 1.106; 1: 250; 2: 0.568; 3: 0.602; 4: 0.585; 5: 0.491; 1: 125; 2: 0.333; 3: 0.341; 4: 0.337; 5: 0.243; 1: 62.5; 2: 0.237; 3: 0.230; 4: 0.234; 5: 0.140; 1: 31.25; 2: 0.166; 3: 0.162; 4: 0.164; 5: 0.070; 1: 0; 2: 0.093; 3: 0.094; 4: 0.094 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human sPECAM1/sCD31 ELISA Kit was designed for the quantitative measurement of Human sPECAM1/sCD31 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL. |
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