Human vimentin,VIM ELISA Kit CSB-E08982h CUSABIO
$50.00
In stock
SKU
CSB-E08982h
Product Details
| Target Name | vimentin |
| Uniprot No. | P08670 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 7.8 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | CTRCT30 ELISA Kit; Epididymis luminal protein 113 ELISA Kit; FLJ36605 ELISA Kit; HEL113 ELISA Kit; VIM ELISA Kit; VIME_HUMAN ELISA Kit; Vimentin ELISA Kit |
| Abbreviation | VIM |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 31.25 pg/mL-2000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cancer |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human VIM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human VIM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human VIM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:100; 2: Average %; 3: 86; 1: Range %; 2: 80-96; 1: 1:200; 2: Average %; 3: 94; 1: Range %; 2: 91-98; 1: 1:400; 2: Average %; 3: 87; 1: Range %; 2: 80-98; 1: 1:800; 2: Average %; 3: 100; 1: Range %; 2: 92-105 |
| Recovery | headers: The recovery of human VIM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human VIM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human VIM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 89; 3: 80-98; 1: EDTA plasma (n=4); 2: 96; 3: 90-106; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 2000 2.654 2.621 2.638 2.532 1000 2.224 2.302 2.263 2.157 500 1.536 1.487 1.512 1.406 250 0.912 0.856 0.884 0.778 125 0.446 0.421 0.434 0.328 62.5 0.256 0.241 0.249 0.143 31.25 0.137 0.145 0.141 0.035 0 0.105 0.107 0.106; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 2000; 9: 2.654; 10: 2.621; 11: 2.638; 12: 2.532; 14: 1000; 15: 2.224; 16: 2.302; 17: 2.263; 18: 2.157; 20: 500; 21: 1.536; 22: 1.487; 23: 1.512; 24: 1.406; 26: 250; 27: 0.912; 28: 0.856; 29: 0.884; 30: 0.778; 32: 125; 33: 0.446; 34: 0.421; 35: 0.434; 36: 0.328; 38: 62.5; 39: 0.256; 40: 0.241; 41: 0.249; 42: 0.143; 44: 31.25; 45: 0.137; 46: 0.145; 47: 0.141; 48: 0.035; 50: 0; 51: 0.105; 52: 0.107; 53: 0.106; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 2000; 2: 2.654; 3: 2.621; 4: 2.638; 5: 2.532; 1: 1000; 2: 2.224; 3: 2.302; 4: 2.263; 5: 2.157; 1: 500; 2: 1.536; 3: 1.487; 4: 1.512; 5: 1.406; 1: 250; 2: 0.912; 3: 0.856; 4: 0.884; 5: 0.778; 1: 125; 2: 0.446; 3: 0.421; 4: 0.434; 5: 0.328; 1: 62.5; 2: 0.256; 3: 0.241; 4: 0.249; 5: 0.143; 1: 31.25; 2: 0.137; 3: 0.145; 4: 0.141; 5: 0.035; 1: 0; 2: 0.105; 3: 0.107; 4: 0.106 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human VIM ELISA Kit was designed for the quantitative measurement of Human VIM protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL. |
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