Monkey Interferon γ ,IFN-γ ELISA Kit CSB-E10049Mo-IS CUSABIO
$50.00
In stock
SKU
CSB-E10049Mo-IS
Product Details
| Target Name | interferon, gamma |
| Uniprot No. | P63310 |
| Species | Monkey |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3.125 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit |
| Abbreviation | IFNG |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range | 6.25 pg/mL-400 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of monkey IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of monkey IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of monkey IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 93; 1: Range %; 2: 89-100; 1: 1:2; 2: Average %; 3: 98; 1: Range %; 2: 91-103; 1: 1:4; 2: Average %; 3: 90; 1: Range %; 2: 83-94; 1: 1:8; 2: Average %; 3: 95; 1: Range %; 2: 87-101 |
| Recovery | headers: The recovery of monkey IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of monkey IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of monkey IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 90; 3: 83-95; 1: EDTA plasma (n=4); 2: 97; 3: 91-101 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 400; 2: 2.543; 3: 2.514; 4: 2.528; 5: 2.432; 1: 200; 2: 1.935; 3: 1.964; 4: 1.949; 5: 1.853; 1: 100; 2: 1.203; 3: 1.228; 4: 1.216; 5: 1.119; 1: 50; 2: 0.552; 3: 0.543; 4: 0.547; 5: 0.451; 1: 25; 2: 0.292; 3: 0.290; 4: 0.291; 5: 0.194; 1: 12.5; 2: 0.222; 3: 0.212; 4: 0.217; 5: 0.121; 1: 6.25; 2: 0.149; 3: 0.156; 4: 0.152; 5: 0.056; 1: 0; 2: 0.100; 3: 0.094; 4: 0.097 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This ELISA kit provides quantitative measurement of interferon-gamma (IFN-γ) in monkey samples, supporting immunological research where this cytokine serves as a central mediator of Th1-driven immune responses. Because IFN-γ is often measured alongside other cytokines to assess immune activation status in nonhuman primate models of infection, vaccination, or transplantation, this kit is designed to address the specific demands of those study designs. Detection Range: The 6.25–400 pg/mL range captures IFN-γ concentrations typical of antigen-stimulated PBMC supernatants and serum from immune-challenged primates, where levels frequently fall between 10 and 300 pg/mL. Unstimulated baseline samples generally require no dilution, while mitogen-stimulated cultures may need predilution. Sensitivity: At 3.125 pg/mL, the kit detects the low-level IFN-γ secretion characteristic of resting or minimally stimulated immune cells, enabling measurement in unstimulated control conditions and early post-vaccination timepoints. Sample Compatibility: Validation across serum, plasma, cell culture supernatants, and tissue homogenates allows researchers to correlate systemic IFN-γ levels with localized tissue responses and ex vivo cellular recall assays within a single study. Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate limited-volume primate specimens and high-throughput screening of multiple stimulation conditions from small PBMC cultures. This kit is particularly suited for NHP vaccine immunogenicity studies, tuberculosis infection models, and graft rejection monitoring. |
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