Canine Procalcitonin(PCT)ELISA Kit CSB-E17400c CUSABIO
$50.00
In stock
SKU
CSB-E17400c
Product Details
| Target Name | Procalcitonin(PCT) |
| Species | Canis lupus familiaris (Dog) (Canis familiaris) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 11.72 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Abbreviation | PCT |
| Sample Types | serum, plasma, cell culture supernates |
| Detection Range | 46.88 pg/mL-3000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of canine PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of canine PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of canine PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 86; 1: Range %; 2: 83-90; 1: 1:2; 2: Average %; 3: 95; 1: Range %; 2: 91-99; 1: 1:4; 2: Average %; 3: 91; 1: Range %; 2: 85-97; 1: 1:8; 2: Average %; 3: 83; 1: Range %; 2: 80-86 |
| Recovery | headers: The recovery of canine PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of canine PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of canine PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 87; 3: 84-93; 1: EDTA plasma (n=4); 2: 104; 3: 101-107; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml OD1 OD2 Average Corrected 3000 2.839 2.901 2.870 2.772 1500 2.341 2.302 2.322 2.224 750 1.602 1.657 1.630 1.532 375 0.920 0.972 0.946 0.848 187.5 0.425 0.408 0.417 0.319 93.75 0.252 0.261 0.257 0.159 46.88 0.144 0.152 0.148 0.050 0 0.099 0.096 0.098; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 9: 3000; 10: 2.839; 11: 2.901; 12: 2.870; 13: 2.772; 16: 1500; 17: 2.341; 18: 2.302; 19: 2.322; 20: 2.224; 23: 750; 24: 1.602; 25: 1.657; 26: 1.630; 27: 1.532; 30: 375; 31: 0.920; 32: 0.972; 33: 0.946; 34: 0.848; 37: 187.5; 38: 0.425; 39: 0.408; 40: 0.417; 41: 0.319; 44: 93.75; 45: 0.252; 46: 0.261; 47: 0.257; 48: 0.159; 51: 46.88; 52: 0.144; 53: 0.152; 54: 0.148; 55: 0.050; 58: 0; 59: 0.099; 60: 0.096; 61: 0.098; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 3000; 2: 2.839; 3: 2.901; 4: 2.870; 5: 2.772; 1: 1500; 2: 2.341; 3: 2.302; 4: 2.322; 5: 2.224; 1: 750; 2: 1.602; 3: 1.657; 4: 1.630; 5: 1.532; 1: 375; 2: 0.920; 3: 0.972; 4: 0.946; 5: 0.848; 1: 187.5; 2: 0.425; 3: 0.408; 4: 0.417; 5: 0.319; 1: 93.75; 2: 0.252; 3: 0.261; 4: 0.257; 5: 0.159; 1: 46.88; 2: 0.144; 3: 0.152; 4: 0.148; 5: 0.050; 1: 0; 2: 0.099; 3: 0.096; 4: 0.098 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Dog PCT ELISA Kit was designed for the quantitative measurement of Dog PCT protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 46.88 pg/mL-3000 pg/mL and the sensitivity is 11.72 pg/mL. |
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