Canine Testosterone (T) ELISA Kit CSB-E06893c CUSABIO
$50.00
In stock
SKU
CSB-E06893c
Product Details
| Target Name | Testosterone (T) |
| Species | Canis lupus familiaris (Dog) (Canis familiaris) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.05 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Abbreviation | T |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.1 ng/mL-20 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Competitive |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<15%; rows: Intra-assay Precision (Precision within an assay): CV%<15%: Intra-assay Precision (Precision within an assay): CV%<15%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<15%: Inter-assay Precision (Precision between assays): CV%<15%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of canine testosterone in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of canine testosterone in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of canine testosterone in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 91; 1: Range %; 2: 86-97; 1: 1:2; 2: Average %; 3: 97; 1: Range %; 2: 92-103; 1: 1:4; 2: Average %; 3: 85; 1: Range %; 2: 81-89; 1: 1:8; 2: Average %; 3: 89; 1: Range %; 2: 83-95 |
| Recovery | headers: The recovery of canine testosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of canine testosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of canine testosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 91; 3: 87-95; 1: EDTA plasma (n=4); 2: 87; 3: 82-93; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average 20 0.232 0.236 0.234 5 0.435 0.436 0.436 1.6 0.611 0.613 0.612 0.4 0.984 0.978 0.981 0.1 1.401 1.403 1.402 0 1.985 1.989 1.987; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 8: 20; 9: 0.232; 10: 0.236; 11: 0.234; 14: 5; 15: 0.435; 16: 0.436; 17: 0.436; 20: 1.6; 21: 0.611; 22: 0.613; 23: 0.612; 26: 0.4; 27: 0.984; 28: 0.978; 29: 0.981; 32: 0.1; 33: 1.401; 34: 1.403; 35: 1.402; 38: 0; 39: 1.985; 40: 1.989; 41: 1.987; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 1: 20; 2: 0.232; 3: 0.236; 4: 0.234; 1: 5; 2: 0.435; 3: 0.436; 4: 0.436; 1: 1.6; 2: 0.611; 3: 0.613; 4: 0.612; 1: 0.4; 2: 0.984; 3: 0.978; 4: 0.981; 1: 0.1; 2: 1.401; 3: 1.403; 4: 1.402; 1: 0; 2: 1.985; 3: 1.989; 4: 1.987 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Testosterone is a steroid hormone that plays important roles in reproductive physiology, sexual development, and secondary sexual characteristics across mammalian species. As the primary male sex hormone, testosterone regulates spermatogenesis, libido, and the development of male reproductive tissues, while also influencing muscle mass, bone density, and behavioral patterns. In veterinary and comparative endocrinology research, testosterone measurements are necessary for studying reproductive health, breeding programs, and hormonal disorders in domestic and wild animal populations. The Canine Testosterone ELISA Kit (CSB-E06893c) provides quantitative measurement of testosterone in canine samples using a competitive assay principle. This kit works with serum, plasma, and tissue homogenates with a detection range of 0.1-20 ng/mL and sensitivity of 0.05 ng/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Measurements are taken at 450 nm wavelength. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This canine testosterone ELISA kit has been used in veterinary endocrine research to quantify testosterone levels in various biological samples. The kit supports hormonal profiling studies and androgen-related research in canine subjects. Applications span reproductive health research and prostatic disease studies where testosterone measurement is needed for understanding hormonal status. • Hormonal profiling - Used alongside estrogen measurements to assess reproductive hormone levels in canine research studies • Prostatic disease research - Applied in conjunction with dihydrotestosterone and estradiol analysis to study hormonal factors in prostate-related conditions • Androgen studies - Used for testosterone quantification in serum and tissue samples as part of comprehensive hormonal assessments • Veterinary endocrine research - Applied in laboratory studies examining hormonal biomarkers alongside other prostatic-specific markers |
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