Mouse Erythropoietin,EPO ELISA Kit CSB-E04539m-IS CUSABIO
$50.00
In stock
SKU
CSB-E04539m-IS
Product Details
| Target Name | erythropoietin |
| Uniprot No. | P07321 |
| Species | Mus musculus (Mouse) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 9.375pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days |
| Associated names | EpoErythropoietin ELISA Kit |
| Abbreviation | EPO |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 18.75pg/mL-1200pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of mouse EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of mouse EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of mouse EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 92; 1: Range %; 2: 85-99; 1: 1:2; 2: Average %; 3: 98; 1: Range %; 2: 93-103; 1: 1:4; 2: Average %; 3: 90; 1: Range %; 2: 85-96; 1: 1:8; 2: Average %; 3: 100; 1: Range %; 2: 95-106 |
| Recovery | headers: The recovery of mouse EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of mouse EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of mouse EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 93; 3: 89-98; 1: EDTA plasma (n=4); 2: 96; 3: 90-103 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 1200; 2: 3.339; 3: 3.234; 4: 3.287; 5: 3.217; 1: 600; 2: 1.837; 3: 1.767; 4: 1.802; 5: 1.732; 1: 300; 2: 0.928; 3: 0.908; 4: 0.918; 5: 0.848; 1: 150; 2: 0.489; 3: 0.469; 4: 0.479; 5: 0.409; 1: 75; 2: 0.313; 3: 0.301; 4: 0.307; 5: 0.237; 1: 37.5; 2: 0.168; 3: 0.179; 4: 0.174; 5: 0.104; 1: 18.75; 2: 0.141; 3: 0.134; 4: 0.138; 5: 0.068; 1: 0; 2: 0.069; 3: 0.071; 4: 0.070 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Mouse EPO ELISA Kit provides quantitative measurement of erythropoietin in Mus musculus samples, supporting immunological research into this hypoxia-responsive cytokine. Because circulating EPO levels fluctuate dramatically between normoxic and hypoxic states, researchers need an assay calibrated to capture both baseline and stress-induced concentrations across diverse tissue contexts. Detection Range: The 18.75–1200 pg/mL range spans the low baseline EPO concentrations typical of healthy mouse serum through the elevated levels observed during acute anemia, hypoxic challenge, or renal injury models, minimizing the need for extensive serial dilutions while accommodating physiological surges. Sensitivity: At 9.375 pg/mL, the assay detects EPO at the low constitutive levels present in normoxic mouse plasma, enabling researchers to establish true baselines before experimental induction of hypoxia or inflammatory stress. Sample Compatibility: Validation across serum, plasma, and tissue homogenates allows direct comparison of systemic circulating EPO with local production in kidney cortex or liver homogenates, supporting studies that distinguish renal versus extrarenal EPO sources within the same experiment. Assay Efficiency: The 1–5 hour protocol requiring only 50–100 µL per well accommodates the limited blood volumes obtainable from individual mice, preserving sample for parallel hematological or cytokine analyses without pooling. This kit is particularly suited for studies of anemia of chronic disease, ischemia-reperfusion injury, and hypoxia-driven immune modulation. |
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