Mouse Interferon γ ,IFN-γ ELISA Kit CSB-E04578m-IS CUSABIO
$50.00
In stock
SKU
CSB-E04578m-IS
Product Details
| Uniprot No. | P01580 |
| Species | Mus musculus (Mouse) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.39 pg/ml |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Ifng ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit |
| Abbreviation | IFNG |
| Sample Types | serum, plasma, tissue homogenates, cell culture supernates. |
| Detection Range | 1.56 pg/ml-100 pg/ml |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 93; 1: Range %; 2: 89-98; 1: 1:2; 2: Average %; 3: 97; 1: Range %; 2: 90-104; 1: 1:4; 2: Average %; 3: 91; 1: Range %; 2: 85-96; 1: 1:8; 2: Average %; 3: 101; 1: Range %; 2: 94-108 |
| Recovery | headers: The recovery of mouse IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of mouse IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of mouse IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 89; 3: 85-95; 1: EDTA plasma (n=4); 2: 97; 3: 91-103 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 100; 2: 3.000; 3: 3.134; 4: 3.067; 5: 2.880; 1: 50; 2: 2.297; 3: 2.178; 4: 2.238; 5: 2.051; 1: 25; 2: 1.259; 3: 1.348; 4: 1.303; 5: 1.116; 1: 12.5; 2: 0.838; 3: 0.829; 4: 0.834; 5: 0.647; 1: 6.25; 2: 0.537; 3: 0.561; 4: 0.549; 5: 0.362; 1: 3.12; 2: 0.367; 3: 0.353; 4: 0.360; 5: 0.173; 1: 1.56; 2: 0.285; 3: 0.274; 4: 0.280; 5: 0.093; 1: 0; 2: 0.189; 3: 0.185; 4: 0.187 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This kit provides quantitative measurement of interferon-gamma (IFN-γ) in mouse samples, supporting immunological research into Th1-mediated responses, infection, and inflammatory disease. Because IFN-γ is often produced transiently and at low concentrations—particularly in unstimulated or early-phase immune responses—this kit is designed to capture biologically meaningful differences across diverse experimental conditions. Detection Range: The 1.56–100 pg/ml range aligns with physiological IFN-γ levels in mouse serum and stimulated splenocyte supernatants, where concentrations typically fall in the low-to-mid pg/ml range. Samples from strongly activated T cells or NK cells (e.g., anti-CD3/CD28 stimulation, LCMV infection models) may require dilution to remain within range. Sensitivity: At 0.39 pg/ml, the kit detects IFN-γ in low-abundance contexts such as naïve mouse serum, early post-infection time points, or conditioned media from small numbers of sorted Th1 or CD8⁺ T cells. Sample Compatibility: Validation across serum, plasma, tissue homogenates, and cell culture supernatants enables paired in vivo/ex vivo study designs—for example, correlating splenic homogenate levels with circulating IFN-γ or restimulation assay readouts. Assay Efficiency: The 1–5 hour protocol and 50–100 µl sample volume accommodate experiments constrained by limited primary cell yields or small-volume bleeds from longitudinal mouse studies. This kit is directly applicable to studies of intracellular infection clearance, graft rejection, and Th1/Th2 polarization. |
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