Mouse Interferon β,IFN-β/IFNB ELISA Kit CSB-E04945m-IS CUSABIO
$50.00
In stock
SKU
CSB-E04945m-IS
Product Details
| Target Name | interferon, beta 1, fibroblast |
| Uniprot No. | P01575 |
| Species | Mus musculus (Mouse) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 15.6pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days |
| Associated names | Ifnb1 ELISA Kit; Ifb ELISA Kit; IfnbInterferon beta ELISA Kit; IFN-beta ELISA Kit |
| Abbreviation | IFNB1 |
| Sample Types | serum, plasma |
| Detection Range | 31.2pg/mL-1000pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-β/IFNB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-β/IFNB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-β/IFNB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 92; 1: Range %; 2: 85-99; 1: 1:2; 2: Average %; 3: 87; 1: Range %; 2: 83-93; 1: 1:4; 2: Average %; 3: 93; 1: Range %; 2: 89-98; 1: 1:8; 2: Average %; 3: 105; 1: Range %; 2: 100-111 |
| Recovery | headers: The recovery of mouse IFN-β/IFNB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of mouse IFN-β/IFNB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of mouse IFN-β/IFNB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 91; 3: 87-96; 1: EDTA plasma (n=4); 2: 97; 3: 90-105 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 1000; 2: 3.179; 3: 3.234; 4: 3.206; 5: 3.064; 1: 500; 2: 1.921; 3: 1.867; 4: 1.894; 5: 1.752; 1: 250; 2: 0.825; 3: 0.828; 4: 0.826; 5: 0.684; 1: 125; 2: 0.459; 3: 0.429; 4: 0.444; 5: 0.302; 1: 62.5; 2: 0.275; 3: 0.281; 4: 0.278; 5: 0.136; 1: 31.2; 2: 0.195; 3: 0.203; 4: 0.199; 5: 0.057; 1: 0; 2: 0.146; 3: 0.138; 4: 0.142 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This ELISA kit provides quantitative measurement of IFNB1 (interferon-beta 1) in mouse serum and plasma, supporting research into type I interferon-mediated innate and adaptive immune responses. Because IFNB1 is often produced in rapid, transient bursts following viral challenge or TLR activation — and circulates at low picomolar concentrations — accurate detection demands both sufficient sensitivity and a well-matched dynamic range. Detection Range: The 31.2–1000 pg/mL range spans the concentrations typically observed in mouse plasma during acute viral infection or poly(I:C) stimulation, where systemic IFNB1 can rise from undetectable baseline levels into the hundreds of pg/mL. Most in vivo samples will fall within range without dilution, though peak responses in certain models may require modest sample dilution. Sensitivity: At 15.6 pg/mL, the kit can detect the low circulating IFNB1 levels present during early infection, subthreshold innate immune priming, or in autoimmune models where chronic low-grade interferon signatures drive pathology. Sample Compatibility: Validation in both serum and plasma allows researchers to align sample collection with pharmacokinetic studies, longitudinal bleeding protocols, or anticoagulant-dependent downstream assays without sacrificing IFNB1 quantification accuracy. Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate serial sampling from individual mice — critical given the small blood volumes and narrow kinetic windows characteristic of IFNB1 response studies. This kit is well suited for investigating antiviral innate immunity, interferon-driven autoimmune pathogenesis such as lupus-like disease, and evaluation of TLR or STING agonist therapeutics. |
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