Recombinant Human C-X-C motif chemokine 3 protein (CXCL3) (Active) CSB-AP000651HU CUSABIO
$354.00
In stock
SKU
CSB-AP000651HU
Product Details
| Uniprot No. | P19876 |
| Alternative Names | C-X-C motif chemokine 3; C-X-C motif chemokine ligand 3; Chemokine (C X C motif) ligand 3; Chemokine (CXC motif) ligand 3; Cinc 2; CINC 2b; Cinc2; CINC2b; CXCL 3; Cxcl3; CXCL3_HUMAN; Cytokine induced neutrophil chemoattractant 2; Dcip1; Dendritic cell inflammatory protein 1; Gm1960; GRO protein gamma; GRO-gamma; GRO-gamma(1-73); GRO-gamma(5-73); GRO3; GRO3 oncogene; GROG; Growth regulated protein gamma; Growth-regulated protein gamma; Macrophage inflammatory protein 2 beta precursor ; Macrophage inflammatory protein 2-beta; Melanoma growth stimulatory activity gamma; Member 3; MGSA gamma; MIP 2b; MIP2-beta; MIP2B; SCYB3; Small inducible cytokine subfamily B |
| Species | Homo sapiens (Human) |
| Source | E.coli |
| Expression Region | 35-107aa |
| Purity | >97% as determined by SDS-PAGE. |
| Endotoxin | Less than 1.0 EU/μg as determined by LAL method. |
| Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml. |
| Tag Info | Tag-Free |
| Form | Lyophilized powder |
| Buffer | Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs | Protein FAQs |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Lead Time | 5-10 business days |
| Target Names | CXCL3 |
| Research Area | Immunology |
| Complete Sequence | ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN |
| Mol. Weight | 7.9 kDa |
| Protein Length | Full Length of Mature Protein |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Datasheet & COA | Please contact us to get it. |
| Description | CXCL3 (GRO-gamma) drives neutrophil recruitment through CXCR2 signaling, making it a critical tool for dissecting chemokine-mediated inflammation and tumor microenvironment biology. This tag-free recombinant human CXCL3, encompassing the full-length mature sequence (residues 35–107), demonstrates confirmed chemotactic activity in a CXCR2-transfected HEK293 bioassay at 10–100 ng/ml, supporting use in chemotaxis and migration assays, CXCR2 signaling pathway studies involving MAPK and NF-κB activation, and in vivo models of neutrophilic inflammation. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound immune cell functional readouts, which provides a suitable basis for sensitive assays such as neutrophil activation, antibody validation by ELISA, and use as a reference standard in cytokine detection platforms. Produced in *E. coli* with purity exceeding 97% by SDS-PAGE, this protein aligns with standards expected in both cell-based functional studies and antibody development workflows. |
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