Mouse Thyroid-Peroxidase,TPO ELISA Kit CSB-E08353m CUSABIO
$50.00
In stock
SKU
CSB-E08353m
Product Details
| Target Name | thyroid peroxidase |
| Uniprot No. | P35419 |
| Species | Mus musculus (Mouse) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 2.5 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Tpo ELISA Kit; Thyroid peroxidase ELISA Kit; TPO ELISA Kit; EC 1.11.1.8 ELISA Kit |
| Abbreviation | TPO |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range | 2.5 ng/mL-40 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cancer |
| Assay Principle | quantitative |
| Measurement | Competitive |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<15%; rows: Intra-assay Precision (Precision within an assay): CV%<15%: Intra-assay Precision (Precision within an assay): CV%<15%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<15%: Inter-assay Precision (Precision between assays): CV%<15%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 90; 1: Range %; 2: 86-103; 1: 1:2; 2: Average %; 3: 89; 1: Range %; 2: 82-97; 1: 1:4; 2: Average %; 3: 95; 1: Range %; 2: 87-100; 1: 1:8; 2: Average %; 3: 90; 1: Range %; 2: 83-99 |
| Recovery | headers: The recovery of mouse TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of mouse TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of mouse TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 94; 3: 87-102; 1: EDTA plasma (n=4); 2: 95; 3: 90-103; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: ng/ml OD1 OD2 Average 40 0.272 0.263 0.268 20 0.525 0.530 0.528 10 0.894 0.874 0.884 5 1.334 1.377 1.356 2.5 2.554 2.528 2.541; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 9: 40; 10: 0.272; 11: 0.263; 12: 0.268; 16: 20; 17: 0.525; 18: 0.530; 19: 0.528; 23: 10; 24: 0.894; 25: 0.874; 26: 0.884; 30: 5; 31: 1.334; 32: 1.377; 33: 1.356; 37: 2.5; 38: 2.554; 39: 2.528; 40: 2.541; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 1: 40; 2: 0.272; 3: 0.263; 4: 0.268; 1: 20; 2: 0.525; 3: 0.530; 4: 0.528; 1: 10; 2: 0.894; 3: 0.874; 4: 0.884; 1: 5; 2: 1.334; 3: 1.377; 4: 1.356; 1: 2.5; 2: 2.554; 3: 2.528; 4: 2.541 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Thyroid peroxidase (TPO) is a key enzyme in thyroid hormone biosynthesis, catalyzing the iodination of tyrosine residues in thyroglobulin and the coupling of iodotyrosines to form thyroxine (T4) and triiodothyronine (T3). TPO sits in the apical membrane of thyroid follicular cells and is required for normal thyroid function and hormone production. TPO serves as a major autoantigen in autoimmune thyroid diseases. Anti-TPO antibodies are important diagnostic markers for conditions such as Hashimoto's thyroiditis and Graves' disease. The Mouse Thyroid-Peroxidase TPO ELISA Kit (CSB-E08353m) provides quantitative measurement of TPO in Mus musculus samples using a competitive assay principle. This kit works with serum, plasma, cell culture supernatants, and tissue homogenates. The detection range spans 2.5 ng/mL to 40 ng/mL with a sensitivity of 2.5 ng/mL. The assay requires 50-100 μL sample volume, measures absorbance at 450 nm wavelength, and can be completed within 1-5 hours. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been used in thyroid-related research to measure thyroid peroxidase levels in biological samples. Applications include autoimmune thyroid disease studies and biomarker research where thyroid peroxidase serves as a key analytical target. • Autoimmune thyroid research: Detection of thyroid peroxidase in serum samples from mouse models to study Hashimoto's thyroiditis and related autoimmune conditions • Thyroid biomarker studies: Quantification of thyroid peroxidase alongside other thyroid-related markers in comprehensive endocrine profiling • Disease model characterization: Measurement of thyroid peroxidase levels in experimental animal studies examining thyroid dysfunction and autoimmune responses |
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