Chicken Tumor necrosis factor α,TNF-α ELISA KIT CSB-E11231Ch CUSABIO
$50.00
In stock
SKU
CSB-E11231Ch
Product Details
| Target Name | Tumor necrosis factor α,TNF-α |
| Species | Gallus gallus (Chicken) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.27 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Abbreviation | TNF-α |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.27 pg/mL-200 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Competitive |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of chicken TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of chicken TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of chicken TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 90; 1: Range %; 2: 85-96; 1: 1:2; 2: Average %; 3: 100; 1: Range %; 2: 95-105; 1: 1:4; 2: Average %; 3: 87; 1: Range %; 2: 82-91; 1: 1:8; 2: Average %; 3: 93; 1: Range %; 2: 86-98 |
| Recovery | headers: The recovery of chicken TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of chicken TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of chicken TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 99; 3: 93-108; 1: EDTA plasma (n=4); 2: 95; 3: 90-99; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average 200 0.173 0.170 0.172 66.7 0.259 0.251 0.255 22.2 0.347 0.367 0.357 7.41 0.452 0.488 0.470 2.47 0.611 0.633 0.622 0.82 0.809 0.855 0.832 0.27 1.033 1.018 1.026 0 1.748 1.707 1.728; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 8: 200; 9: 0.173; 10: 0.170; 11: 0.172; 14: 66.7; 15: 0.259; 16: 0.251; 17: 0.255; 20: 22.2; 21: 0.347; 22: 0.367; 23: 0.357; 26: 7.41; 27: 0.452; 28: 0.488; 29: 0.470; 32: 2.47; 33: 0.611; 34: 0.633; 35: 0.622; 38: 0.82; 39: 0.809; 40: 0.855; 41: 0.832; 44: 0.27; 45: 1.033; 46: 1.018; 47: 1.026; 50: 0; 51: 1.748; 52: 1.707; 53: 1.728; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 1: 200; 2: 0.173; 3: 0.170; 4: 0.172; 1: 66.7; 2: 0.259; 3: 0.251; 4: 0.255; 1: 22.2; 2: 0.347; 3: 0.367; 4: 0.357; 1: 7.41; 2: 0.452; 3: 0.488; 4: 0.470; 1: 2.47; 2: 0.611; 3: 0.633; 4: 0.622; 1: 0.82; 2: 0.809; 3: 0.855; 4: 0.832; 1: 0.27; 2: 1.033; 3: 1.018; 4: 1.026; 1: 0; 2: 1.748; 3: 1.707; 4: 1.728 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Chicken TNF-α ELISA Kit was designed for the quantitative measurement of Chicken TNF-α protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.27 pg/mL-200 pg/mL and the sensitivity is 0.27 pg/mL. |
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