Mouse Vascular Endothelial cell Growth Factor B,VEGF-B ELISA kit CSB-E07365m CUSABIO
$50.00
In stock
SKU
CSB-E07365m
Product Details
| Target Name | vascular endothelial growth factor B |
| Uniprot No. | P49766 |
| Species | Mus musculus (Mouse) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.195 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Vegfb ELISA kit; VrfVascular endothelial growth factor B ELISA kit; VEGF-B ELISA kit; VEGF-related factor ELISA kit; VRF ELISA kit |
| Abbreviation | VEGFB |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range | 7.8 pg/mL-500 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cancer |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGF-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGF-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGF-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 97; 1: Range %; 2: 92-102; 1: 1:2; 2: Average %; 3: 91; 1: Range %; 2: 84-97; 1: 1:4; 2: Average %; 3: 90; 1: Range %; 2: 87-94; 1: 1:8; 2: Average %; 3: 100; 1: Range %; 2: 95-103 |
| Recovery | headers: The recovery of mouse VEGF-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of mouse VEGF-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of mouse VEGF-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 90; 3: 87-93; 1: EDTA plasma (n=4); 2: 93; 3: 90-96; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 500 2.743 2.763 2.753 2.589 250 2.405 2.435 2.420 2.256 125 1.756 1.796 1.776 1.612 62.5 0.978 1.016 0.997 0.833 31.2 0.501 0.535 0.518 0.354 15.6 0.351 0.359 0.355 0.191 0.78 0.237 0.255 0.246 0.082 0 0.162 0.165 0.164; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 500; 9: 2.743; 10: 2.763; 11: 2.753; 12: 2.589; 14: 250; 15: 2.405; 16: 2.435; 17: 2.420; 18: 2.256; 20: 125; 21: 1.756; 22: 1.796; 23: 1.776; 24: 1.612; 26: 62.5; 27: 0.978; 28: 1.016; 29: 0.997; 30: 0.833; 32: 31.2; 33: 0.501; 34: 0.535; 35: 0.518; 36: 0.354; 38: 15.6; 39: 0.351; 40: 0.359; 41: 0.355; 42: 0.191; 44: 0.78; 45: 0.237; 46: 0.255; 47: 0.246; 48: 0.082; 50: 0; 51: 0.162; 52: 0.165; 53: 0.164; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 500; 2: 2.743; 3: 2.763; 4: 2.753; 5: 2.589; 1: 250; 2: 2.405; 3: 2.435; 4: 2.420; 5: 2.256; 1: 125; 2: 1.756; 3: 1.796; 4: 1.776; 5: 1.612; 1: 62.5; 2: 0.978; 3: 1.016; 4: 0.997; 5: 0.833; 1: 31.2; 2: 0.501; 3: 0.535; 4: 0.518; 5: 0.354; 1: 15.6; 2: 0.351; 3: 0.359; 4: 0.355; 5: 0.191; 1: 0.78; 2: 0.237; 3: 0.255; 4: 0.246; 5: 0.082; 1: 0; 2: 0.162; 3: 0.165; 4: 0.164 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Mouse VEGFB ELISA Kit was designed for the quantitative measurement of Mouse VEGFB protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 0.195 pg/mL . |
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