Chicken adrenocorticotropic hormone (ACTH) ELISA kit CSB-E14373C CUSABIO
$50.00
In stock
SKU
CSB-E14373C
Product Details
| Target Name | adrenocorticotropic hormone (ACTH) |
| Species | Gallus gallus (Chicken) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.125 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Abbreviation | ACTH |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.25 pg/mL-50 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Metabolism |
| Assay Principle | quantitative |
| Measurement | Competitive |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<15%; rows: Intra-assay Precision (Precision within an assay): CV%<15%: Intra-assay Precision (Precision within an assay): CV%<15%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<15%: Inter-assay Precision (Precision between assays): CV%<15%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of chicken ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of chicken ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of chicken ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 96; 1: Range %; 2: 91-102; 1: 1:2; 2: Average %; 3: 91; 1: Range %; 2: 97-95; 1: 1:4; 2: Average %; 3: 89; 1: Range %; 2: 84-93; 1: 1:8; 2: Average %; 3: 98; 1: Range %; 2: 92-104 |
| Recovery | headers: The recovery of chicken ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of chicken ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of chicken ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 92; 3: 88-96; 1: EDTA plasma (n=4); 2: 97; 3: 91-103; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average 50 0.176 0.174 0.175 12.5 0.321 0.316 0.319 4 0.534 0.514 0.524 1 0.888 0.857 0.873 0.25 1.432 1.324 1.378 0 1.986 1.905 1.946; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 8: 50; 9: 0.176; 10: 0.174; 11: 0.175; 14: 12.5; 15: 0.321; 16: 0.316; 17: 0.319; 20: 4; 21: 0.534; 22: 0.514; 23: 0.524; 26: 1; 27: 0.888; 28: 0.857; 29: 0.873; 32: 0.25; 33: 1.432; 34: 1.324; 35: 1.378; 38: 0; 39: 1.986; 40: 1.905; 41: 1.946; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 1: 50; 2: 0.176; 3: 0.174; 4: 0.175; 1: 12.5; 2: 0.321; 3: 0.316; 4: 0.319; 1: 4; 2: 0.534; 3: 0.514; 4: 0.524; 1: 1; 2: 0.888; 3: 0.857; 4: 0.873; 1: 0.25; 2: 1.432; 3: 1.324; 4: 1.378; 1: 0; 2: 1.986; 3: 1.905; 4: 1.946 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Chicken ACTH ELISA Kit was designed for the quantitative measurement of Chicken ACTH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.25 pg/mL-50 pg/mL and the sensitivity is 0.125 pg/mL. |
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