Mouse adiponectin,ADP ELISA Kit CSB-E07272m CUSABIO
$50.00
In stock
SKU
CSB-E07272m
Product Details
| Target Name | adiponectin, C1Q and collagen domain containing |
| Uniprot No. | Q60994 |
| Species | Mus musculus (Mouse) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.039 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Adipoq ELISA Kit; Acdc ELISA Kit; Acrp30 ELISA Kit; Apm1 ELISA Kit; Adiponectin ELISA Kit; 30 kDa adipocyte complement-related protein ELISA Kit; Adipocyte complement-related 30 kDa protein ELISA Kit; ACRP30 ELISA Kit; Adipocyte ELISA Kit; C1q and collagen domain-containing protein ELISA Kit; Adipocyte-specific protein AdipoQ ELISA Kit |
| Abbreviation | ADP |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates, cell lysates |
| Detection Range | 0.156 ng/mL-10 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cardiovascular |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Typical Data | These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | CUSABIO's mouse adiponectin (ADP) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative determination of adiponectin concentrations in serum, plasma, cell culture supernates, tissue homogenates, and cell lysates. This assay exclusively recognizes mouse adiponectin. The quantitative sandwich ELISA technique of this kit is based on adiponectin antibody-adiponectin antigen interactions and an HRP colorimetric detection system to detect the levels of adiponectin in samples. The intensity of the color is positively proportional to the amount of bound adiponectin in the initial step. Adiponectin, the most abundant plasma adipokine, plays an important role in the regulation of glucose, lipid metabolism, and insulin sensitivity through its anti-inflammatory, anti-fibrotic, anti-oxidant, angiogenic, and vasodilatory properties. Through its receptor AdipoR1 and AdipoR2, adiponectin signaling is involved in numerous essential regulatory cellular signaling mechanisms including AMPK, p38/MAPK signaling, STAT3, and lipid metabolic pathways. Weight loss or caloric restriction results in increasing adiponectin levels, and this increase is related to enhanced insulin sensitivity. Low plasma levels of adiponectin, also known as hypoadiponectinemia, are linked to the increased prevalence of obesity-associated diseases, including type 2 diabetes, coronary heart disease, and hypertension. |
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