Mouse apolipoprotein E (Apo-E) ELISA Kit CSB-E09750m CUSABIO
$50.00
In stock
SKU
CSB-E09750m
Product Details
| Target Name | apolipoprotein E |
| Uniprot No. | P08226 |
| Species | Mus musculus (Mouse) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 1.56 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | ApoeApolipoprotein E ELISA kit; Apo-E ELISA kit |
| Abbreviation | APOE |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range | 6.25 ng/mL-400 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cardiovascular |
| Assay Principle | quantitative |
| Measurement | Competitive |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:100; 2: Average %; 3: 99; 1: Range %; 2: 96-101; 1: 1:200; 2: Average %; 3: 85; 1: Range %; 2: 81-89; 1: 1:400; 2: Average %; 3: 100; 1: Range %; 2: 97-103; 1: 1:800; 2: Average %; 3: 83; 1: Range %; 2: 80-86 |
| Recovery | headers: The recovery of mouse Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of mouse Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of mouse Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 97; 3: 93-100; 1: EDTA plasma (n=4); 2: 99; 3: 95-104; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average 400 0.157 0.159 0.158 200 0.249 0.269 0.259 100 0.432 0.441 0.437 50 0.643 0.672 0.658 25 0.955 0.984 0.970 12.5 1.199 1.172 1.186 6.25 1.568 1.572 1.570 0 2.573 2.591 2.582; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 8: 400; 9: 0.157; 10: 0.159; 11: 0.158; 14: 200; 15: 0.249; 16: 0.269; 17: 0.259; 20: 100; 21: 0.432; 22: 0.441; 23: 0.437; 26: 50; 27: 0.643; 28: 0.672; 29: 0.658; 32: 25; 33: 0.955; 34: 0.984; 35: 0.970; 38: 12.5; 39: 1.199; 40: 1.172; 41: 1.186; 44: 6.25; 45: 1.568; 46: 1.572; 47: 1.570; 50: 0; 51: 2.573; 52: 2.591; 53: 2.582; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 1: 400; 2: 0.157; 3: 0.159; 4: 0.158; 1: 200; 2: 0.249; 3: 0.269; 4: 0.259; 1: 100; 2: 0.432; 3: 0.441; 4: 0.437; 1: 50; 2: 0.643; 3: 0.672; 4: 0.658; 1: 25; 2: 0.955; 3: 0.984; 4: 0.970; 1: 12.5; 2: 1.199; 3: 1.172; 4: 1.186; 1: 6.25; 2: 1.568; 3: 1.572; 4: 1.570; 1: 0; 2: 2.573; 3: 2.591; 4: 2.582 |
| Materials provided | A 96-well Assay plate --The 96-well plate has been pre-coated with ApoE. Two pieces of Standard (Freeze-dried)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve. HRP-conjugated ApoE antibody (100 x concentrate) (1 x 6 μl) --Act as the detection antibody. HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated ApoE antibody solution. Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration. Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances. TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) An Instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm. An incubator that can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Mouse APOE ELISA Kit was designed for the quantitative measurement of Mouse APOE protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL. |
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