Mouse regulated on activation in normal T-cell expressed and secreted,RANTES ELISA kit CSB-E09256m-IS CUSABIO
$50.00
In stock
SKU
CSB-E09256m-IS
Product Details
| Uniprot No. | P30882 |
| Species | Mus musculus (Mouse) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3.9 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Associated names | Ccl5 ELISA Kit; Scya5C-C motif chemokine 5 ELISA Kit; MuRantes ELISA Kit; SIS-delta ELISA Kit; Small-inducible cytokine A5 ELISA Kit; T-cell-specific protein RANTES ELISA Kit |
| Abbreviation | CCL5 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 7.8 pg/mL-500 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of mouse RANTES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of mouse RANTES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of mouse RANTES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 106; 1: Range %; 2: 101-112; 1: 1:2; 2: Average %; 3: 95; 1: Range %; 2: 90-100; 1: 1:4; 2: Average %; 3: 92; 1: Range %; 2: 85-99; 1: 1:8; 2: Average %; 3: 99; 1: Range %; 2: 94-105 |
| Recovery | headers: The recovery of mouse RANTES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of mouse RANTES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of mouse RANTES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 94; 3: 89-98; 1: EDTA plasma (n=4); 2: 88; 3: 81-96 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 500; 2: 2.903; 3: 2.976; 4: 2.939; 5: 2.762; 1: 250; 2: 2.375; 3: 2.497; 4: 2.436; 5: 2.259; 1: 125; 2: 1.790; 3: 1.698; 4: 1.744; 5: 1.567; 1: 62.5; 2: 0.903; 3: 0.929; 4: 0.916; 5: 0.738; 1: 31.2; 2: 0.532; 3: 0.561; 4: 0.547; 5: 0.369; 1: 15.6; 2: 0.384; 3: 0.393; 4: 0.388; 5: 0.211; 1: 7.8; 2: 0.278; 3: 0.287; 4: 0.282; 5: 0.105; 1: 0; 2: 0.180; 3: 0.175; 4: 0.177 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Mouse CCL5 (RANTES) ELISA Kit provides quantitative measurement of CCL5 in Mus musculus samples, supporting immunology research focused on chemokine-driven leukocyte recruitment and inflammatory signaling. Because CCL5 concentrations can vary dramatically between resting and inflamed states — often spanning two orders of magnitude — this kit is designed to capture that dynamic range while remaining sensitive to baseline levels. Detection Range: The 7.8–500 pg/mL range covers both constitutive CCL5 levels in healthy mouse serum (typically 20–100 pg/mL) and the elevated concentrations seen during acute inflammation or viral infection. Most stimulated samples will fall within range without dilution, though LPS-challenged tissue homogenates may require 2–4× dilution. Sensitivity: At 3.9 pg/mL, the kit detects CCL5 in low-expression contexts such as naïve T cell supernatants, early post-infection serum, or unstimulated tissue, where concentrations often fall below 10 pg/mL. Sample Compatibility: Validation across serum, plasma, and tissue homogenates (50–100 µL) enables paired systemic-versus-local CCL5 profiling — for example, comparing circulating plasma levels with lung or liver homogenate concentrations within the same animal. Assay Efficiency: The 1–5 hour protocol and low 50–100 µL sample volume accommodate studies using serial bleeds from individual mice or limited primary cell-conditioned media, preserving sample for parallel cytokine measurements. This kit is particularly suited for studies of allergic airway inflammation, atherosclerotic plaque progression, and T cell-mediated antiviral immunity. |
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