Mouse serum amyloid A1 (SAA1) ELISA kit CSB-EL020656MO CUSABIO
$50.00
In stock
SKU
CSB-EL020656MO
Product Details
| Uniprot No. | P05366 |
| Species | Mus musculus (Mouse) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.78 ng/ml |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Saa1 ELISA Kit; Serum amyloid A-1 protein ELISA Kit |
| Abbreviation | SAA1 |
| Sample Types | serum, plasma,tissue homogenates |
| Detection Range | 3.12 ng/ml - 200 ng/ml |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cardiovascular |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of mouse SAA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of mouse SAA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of mouse SAA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:200; 2: Average %; 3: 90; 1: Range %; 2: 85-96; 1: 1:400; 2: Average %; 3: 92; 1: Range %; 2: 89-95; 1: 1:800; 2: Average %; 3: 91; 1: Range %; 2: 87-96; 1: 1:1600; 2: Average %; 3: 95; 1: Range %; 2: 86-101 |
| Recovery | headers: The recovery of mouse SAA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of mouse SAA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of mouse SAA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 88; 3: 82-94; 1: EDTA plasma (n=4); 2: 96; 3: 90-101 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 200; 2: 2.200; 3: 2.100; 4: 2.150; 5: 2.055; 1: 100; 2: 1.405; 3: 1.305; 4: 1.355; 5: 1.260; 1: 50; 2: 0.802; 3: 0.782; 4: 0.792; 5: 0.697; 1: 25; 2: 0.435; 3: 0.425; 4: 0.430; 5: 0.335; 1: 12.5; 2: 0.304; 3: 0.294; 4: 0.299; 5: 0.204; 1: 6.25; 2: 0.201; 3: 0.191; 4: 0.196; 5: 0.101; 1: 3.12; 2: 0.147; 3: 0.146; 4: 0.147; 5: 0.052; 1: 0; 2: 0.095; 3: 0.094; 4: 0.095 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | The mouse SAA1 ELISA Kit is used to quantitatively measure mouse SAA1 concentrations in serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of SAA1 in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader. SAA1 is known as a major precursor of amyloid A and plays an important role in lipid metabolism, antibacterial functions, inflammation regulation, and tumor pathogenesis. SAA1 has been shown to upregulate the expression of various inflammatory mediators such as cell adhesion molecules, cytokines, chemokines, and ROS, as well as pro-angiogenic molecules in leukocytes, fibroblasts, and endothelial cells. SAA1 being a major acute-phase protein is increasingly used as a reliable predictor of coronary artery disease (CAD). Elevation of SAA1 expression, synthesis, and release to the circulation is induced by inflammation, trauma, or stress that activates the acute-phase response (APR). |
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