Rat Caspase 3,Casp-3 ELISA Kit CSB-E08857r CUSABIO
$50.00
In stock
SKU
CSB-E08857r
Product Details
| Target Name | caspase 3, apoptosis-related cysteine peptidase |
| Uniprot No. | P55213 |
| Species | Rattus norvegicus (Rat) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.078 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Casp3 ELISA Kit; Cpp32Caspase-3 ELISA Kit; CASP-3 ELISA Kit; EC 3.4.22.56 ELISA Kit; Apopain ELISA Kit; Cysteine protease CPP32 ELISA Kit; CPP-32 ELISA Kit; IRP ELISA Kit; LICE ELISA Kit; Protein Yama ELISA Kit; SREBP cleavage activity 1 ELISA Kit; SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12] ELISA Kit |
| Abbreviation | CASP3 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.312 ng/mL-20 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cell Biology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 84; 1: Range %; 2: 80-92; 1: 1:2; 2: Average %; 3: 98; 1: Range %; 2: 91-105; 1: 1:4; 2: Average %; 3: 99; 1: Range %; 2: 92-110; 1: 1:8; 2: Average %; 3: 91; 1: Range %; 2: 86-98 |
| Recovery | headers: The recovery of rat Casp-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of rat Casp-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of rat Casp-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 89-98; 1: EDTA plasma (n=4); 2: 96; 3: 90-100; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 20 2.915 2.903 2.909 2.828 10 2.321 2.283 2.302 2.221 5 1.467 1.444 1.456 1.375 2.5 0.727 0.706 0.717 0.636 1.25 0.365 0.359 0.362 0.281 0.625 0.216 0.206 0.211 0.130 0.312 0.137 0.129 0.133 0.052 0 0.084 0.078 0.081; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 20; 9: 2.915; 10: 2.903; 11: 2.909; 12: 2.828; 14: 10; 15: 2.321; 16: 2.283; 17: 2.302; 18: 2.221; 20: 5; 21: 1.467; 22: 1.444; 23: 1.456; 24: 1.375; 26: 2.5; 27: 0.727; 28: 0.706; 29: 0.717; 30: 0.636; 32: 1.25; 33: 0.365; 34: 0.359; 35: 0.362; 36: 0.281; 38: 0.625; 39: 0.216; 40: 0.206; 41: 0.211; 42: 0.130; 44: 0.312; 45: 0.137; 46: 0.129; 47: 0.133; 48: 0.052; 50: 0; 51: 0.084; 52: 0.078; 53: 0.081; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 20; 2: 2.915; 3: 2.903; 4: 2.909; 5: 2.828; 1: 10; 2: 2.321; 3: 2.283; 4: 2.302; 5: 2.221; 1: 5; 2: 1.467; 3: 1.444; 4: 1.456; 5: 1.375; 1: 2.5; 2: 0.727; 3: 0.706; 4: 0.717; 5: 0.636; 1: 1.25; 2: 0.365; 3: 0.359; 4: 0.362; 5: 0.281; 1: 0.625; 2: 0.216; 3: 0.206; 4: 0.211; 5: 0.130; 1: 0.312; 2: 0.137; 3: 0.129; 4: 0.133; 5: 0.052; 1: 0; 2: 0.084; 3: 0.078; 4: 0.081 |
| Materials provided | A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Rat CASP3 antibody. Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled CASP3 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody. One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution. One vial Sample Diluent (50 ml/bottle) ---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubating. An instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator can provide stable incubation conditions up to 37°C±5° Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Rat CASP3 ELISA Kit was designed for the quantitative measurement of Rat CASP3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL . |
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