Rat Insulin-like growth factor binding protein 1,IGFBP-1 ELISA Kit CSB-E07349r CUSABIO
$50.00
In stock
SKU
CSB-E07349r
Product Details
| Target Name | insulin-like growth factor binding protein 1 |
| Uniprot No. | P21743 |
| Species | Rattus norvegicus (Rat) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3.9 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Igfbp1 ELISA Kit; Igfbp-1 ELISA Kit; Insulin-like growth factor-binding protein 1 ELISA Kit; IBP-1 ELISA Kit; IGF-binding protein 1 ELISA Kit; IGFBP-1 ELISA Kit |
| Abbreviation | IGFBP1 |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range | 15.6 pg/mL-1000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of rat IGFBP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of rat IGFBP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of rat IGFBP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:100; 2: Average %; 3: 87; 1: Range %; 2: 80-94; 1: 1:200; 2: Average %; 3: 97; 1: Range %; 2: 91-105; 1: 1:400; 2: Average %; 3: 88; 1: Range %; 2: 81-95; 1: 1:800; 2: Average %; 3: 100; 1: Range %; 2: 95-94 |
| Recovery | headers: The recovery of rat IGFBP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of rat IGFBP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of rat IGFBP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 94; 3: 89-99; 1: EDTA plasma (n=4); 2: 92; 3: 87-97; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 1000 2.142 2.287 2.215 2.030 500 1.882 1.897 1.890 1.705 250 1.297 1.324 1.311 1.126 125 0.803 0.815 0.809 0.624 62.5 0.492 0.506 0.499 0.314 31.2 0.360 0.387 0.374 0.189 15.6 0.273 0.288 0.281 0.096 0 0.184 0.186 0.185; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 1000; 9: 2.142; 10: 2.287; 11: 2.215; 12: 2.030; 14: 500; 15: 1.882; 16: 1.897; 17: 1.890; 18: 1.705; 20: 250; 21: 1.297; 22: 1.324; 23: 1.311; 24: 1.126; 26: 125; 27: 0.803; 28: 0.815; 29: 0.809; 30: 0.624; 32: 62.5; 33: 0.492; 34: 0.506; 35: 0.499; 36: 0.314; 38: 31.2; 39: 0.360; 40: 0.387; 41: 0.374; 42: 0.189; 44: 15.6; 45: 0.273; 46: 0.288; 47: 0.281; 48: 0.096; 50: 0; 51: 0.184; 52: 0.186; 53: 0.185; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 1000; 2: 2.142; 3: 2.287; 4: 2.215; 5: 2.030; 1: 500; 2: 1.882; 3: 1.897; 4: 1.890; 5: 1.705; 1: 250; 2: 1.297; 3: 1.324; 4: 1.311; 5: 1.126; 1: 125; 2: 0.803; 3: 0.815; 4: 0.809; 5: 0.624; 1: 62.5; 2: 0.492; 3: 0.506; 4: 0.499; 5: 0.314; 1: 31.2; 2: 0.360; 3: 0.387; 4: 0.374; 5: 0.189; 1: 15.6; 2: 0.273; 3: 0.288; 4: 0.281; 5: 0.096; 1: 0; 2: 0.184; 3: 0.186; 4: 0.185 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Rat IGFBP1 ELISA Kit was designed for the quantitative measurement of Rat IGFBP1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL. |
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