Rat Insulin-like growth factor binding protein 3,IGFBP-3 ELISA kit CSB-E07353r CUSABIO
$50.00
In stock
SKU
CSB-E07353r
Product Details
| Uniprot No. | P15473 |
| Species | Rattus norvegicus (Rat) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.06 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Igfbp3 ELISA Kit; Igfbp-3 ELISA Kit; Insulin-like growth factor-binding protein 3 ELISA Kit; IBP-3 ELISA Kit; IGF-binding protein 3 ELISA Kit; IGFBP-3 ELISA Kit |
| Abbreviation | IGFBP3 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.24 ng/mL-15 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of rat IGFBP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of rat IGFBP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of rat IGFBP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:20; 2: Average %; 3: 84; 1: Range %; 2: 80-92; 1: 1:40; 2: Average %; 3: 90; 1: Range %; 2: 85-95; 1: 1:80; 2: Average %; 3: 97; 1: Range %; 2: 92-107; 1: 1:160; 2: Average %; 3: 106; 1: Range %; 2: 101-112 |
| Recovery | headers: The recovery of rat IGFBP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of rat IGFBP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of rat IGFBP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 90; 3: 86-97; 1: EDTA plasma (n=4); 2: 93; 3: 88-101 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 15; 2: 3.631; 3: 3.417; 4: 3.524; 5: 3.441; 1: 7.5; 2: 3.033; 3: 3.063; 4: 3.048; 5: 2.965; 1: 3.75; 2: 2.255; 3: 2.223; 4: 2.239; 5: 2.156; 1: 1.88; 2: 1.221; 3: 1.286; 4: 1.254; 5: 1.170; 1: 0.94; 2: 0.691; 3: 0.677; 4: 0.684; 5: 0.601; 1: 0.47; 2: 0.434; 3: 0.430; 4: 0.432; 5: 0.349; 1: 0.24; 2: 0.268; 3: 0.274; 4: 0.271; 5: 0.188; 1: 0; 2: 0.085; 3: 0.081; 4: 0.083 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
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