Rat Interleukin 23,IL-23 ELISA Kit CSB-E08462r CUSABIO
$50.00
In stock
SKU
CSB-E08462r
Product Details
| Target Name | Interleukin 23,IL-23 |
| Species | Rattus norvegicus (Rat) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.78 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Abbreviation | IL-23 |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range | 3.12 pg/mL-200 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 92; 1: Range %; 2: 89-94; 1: 1:2; 2: Average %; 3: 104; 1: Range %; 2: 100-107; 1: 1:4; 2: Average %; 3: 92; 1: Range %; 2: 87-95 |
| Recovery | headers: The recovery of rat IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of rat IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of rat IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 93; 3: 89-95; 1: EDTA plasma (n=4); 2: 98; 3: 94-100; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 200 2.147 2.112 2.130 1.998 100 1.851 1.776 1.814 1.682 50 1.308 1.288 1.298 1.166 25 0.903 0.898 0.901 0.769 12.5 0.621 0.614 0.618 0.486 6.25 0.375 0.359 0.367 0.235 3.12 0.236 0.224 0.230 0.098 0 0.134 0.130 0.132; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 200; 9: 2.147; 10: 2.112; 11: 2.130; 12: 1.998; 14: 100; 15: 1.851; 16: 1.776; 17: 1.814; 18: 1.682; 20: 50; 21: 1.308; 22: 1.288; 23: 1.298; 24: 1.166; 26: 25; 27: 0.903; 28: 0.898; 29: 0.901; 30: 0.769; 32: 12.5; 33: 0.621; 34: 0.614; 35: 0.618; 36: 0.486; 38: 6.25; 39: 0.375; 40: 0.359; 41: 0.367; 42: 0.235; 44: 3.12; 45: 0.236; 46: 0.224; 47: 0.230; 48: 0.098; 50: 0; 51: 0.134; 52: 0.130; 53: 0.132; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 200; 2: 2.147; 3: 2.112; 4: 2.130; 5: 1.998; 1: 100; 2: 1.851; 3: 1.776; 4: 1.814; 5: 1.682; 1: 50; 2: 1.308; 3: 1.288; 4: 1.298; 5: 1.166; 1: 25; 2: 0.903; 3: 0.898; 4: 0.901; 5: 0.769; 1: 12.5; 2: 0.621; 3: 0.614; 4: 0.618; 5: 0.486; 1: 6.25; 2: 0.375; 3: 0.359; 4: 0.367; 5: 0.235; 1: 3.12; 2: 0.236; 3: 0.224; 4: 0.230; 5: 0.098; 1: 0; 2: 0.134; 3: 0.130; 4: 0.132 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Rat IL-23 ELISA Kit was designed for the quantitative measurement of Rat IL-23 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL . |
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