Rat Neuron-specific enolase,NSE ELISA Kit CSB-E07963r CUSABIO
$50.00
In stock
SKU
CSB-E07963r
Product Details
| Target Name | enolase 2 (gamma, neuronal) |
| Uniprot No. | P07323 |
| Species | Rattus norvegicus (Rat) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.156 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Eno2 ELISA Kit; Eno-2 ELISA Kit; Gamma-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 2 ELISA Kit; Neural enolase ELISA Kit; Neuron-specific enolase ELISA Kit; NSE ELISA Kit |
| Abbreviation | ENO2 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.625 ng/mL-40 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Metabolism |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of rat NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of rat NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of rat NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 89; 1: Range %; 2: 85-94; 1: 1:2; 2: Average %; 3: 100; 1: Range %; 2: 97-104; 1: 1:4; 2: Average %; 3: 90; 1: Range %; 2: 87-94; 1: 1:8; 2: Average %; 3: 100; 1: Range %; 2: 95-103 |
| Recovery | headers: The recovery of rat NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of rat NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of rat NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 91-98; 1: EDTA plasma (n=4); 2: 95; 3: 92-97; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 40 2.260 2.232 2.246 2.078 20 1.454 1.486 1.470 1.302 10 0.920 0.928 0.924 0.756 5 0.654 0.663 0.659 0.491 2.5 0.403 0.398 0.401 0.233 1.25 0.322 0.314 0.318 0.150 0.625 0.266 0.257 0.262 0.094 0 0.170 0.166 0.168; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 40; 9: 2.260; 10: 2.232; 11: 2.246; 12: 2.078; 14: 20; 15: 1.454; 16: 1.486; 17: 1.470; 18: 1.302; 20: 10; 21: 0.920; 22: 0.928; 23: 0.924; 24: 0.756; 26: 5; 27: 0.654; 28: 0.663; 29: 0.659; 30: 0.491; 32: 2.5; 33: 0.403; 34: 0.398; 35: 0.401; 36: 0.233; 38: 1.25; 39: 0.322; 40: 0.314; 41: 0.318; 42: 0.150; 44: 0.625; 45: 0.266; 46: 0.257; 47: 0.262; 48: 0.094; 50: 0; 51: 0.170; 52: 0.166; 53: 0.168; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 40; 2: 2.260; 3: 2.232; 4: 2.246; 5: 2.078; 1: 20; 2: 1.454; 3: 1.486; 4: 1.470; 5: 1.302; 1: 10; 2: 0.920; 3: 0.928; 4: 0.924; 5: 0.756; 1: 5; 2: 0.654; 3: 0.663; 4: 0.659; 5: 0.491; 1: 2.5; 2: 0.403; 3: 0.398; 4: 0.401; 5: 0.233; 1: 1.25; 2: 0.322; 3: 0.314; 4: 0.318; 5: 0.150; 1: 0.625; 2: 0.266; 3: 0.257; 4: 0.262; 5: 0.094; 1: 0; 2: 0.170; 3: 0.166; 4: 0.168 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Rat ENO2 ELISA Kit was designed for the quantitative measurement of Rat ENO2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL . |
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