Rat Sclerostin(SOST) ELISA kit CSB-EL022415RA CUSABIO
$50.00
In stock
SKU
CSB-EL022415RA
Product Details
| Target Name | sclerosteosis |
| Uniprot No. | Q99P67 |
| Species | Rattus norvegicus (Rat) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 4.68 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Sost ELISA Kit; Sclerostin ELISA Kit |
| Abbreviation | SOST |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 18.75 pg/mL-1200 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of rat SOST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of rat SOST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of rat SOST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 97; 1: Range %; 2: 93-101; 1: 1:2; 2: Average %; 3: 92; 1: Range %; 2: 86-99; 1: 1:4; 2: Average %; 3: 90; 1: Range %; 2: 88-94; 1: 1:8; 2: Average %; 3: 101; 1: Range %; 2: 96-103 |
| Recovery | headers: The recovery of rat SOST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of rat SOST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of rat SOST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 97; 3: 94-103; 1: EDTA plasma (n=4); 2: 105; 3: 105-109; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 1200 2.362 2.545 2.454 2.316 600 1.588 1.686 1.637 1.499 300 1.123 1.102 1.113 0.975 150 0.767 0.744 0.756 0.618 75 0.520 0.546 0.533 0.395 37.5 0.304 0.324 0.314 0.176 18.75 0.248 0.241 0.245 0.107 0 0.136 0.140 0.138; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 1200; 9: 2.362; 10: 2.545; 11: 2.454; 12: 2.316; 14: 600; 15: 1.588; 16: 1.686; 17: 1.637; 18: 1.499; 20: 300; 21: 1.123; 22: 1.102; 23: 1.113; 24: 0.975; 26: 150; 27: 0.767; 28: 0.744; 29: 0.756; 30: 0.618; 32: 75; 33: 0.520; 34: 0.546; 35: 0.533; 36: 0.395; 38: 37.5; 39: 0.304; 40: 0.324; 41: 0.314; 42: 0.176; 44: 18.75; 45: 0.248; 46: 0.241; 47: 0.245; 48: 0.107; 50: 0; 51: 0.136; 52: 0.140; 53: 0.138; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 1200; 2: 2.362; 3: 2.545; 4: 2.454; 5: 2.316; 1: 600; 2: 1.588; 3: 1.686; 4: 1.637; 5: 1.499; 1: 300; 2: 1.123; 3: 1.102; 4: 1.113; 5: 0.975; 1: 150; 2: 0.767; 3: 0.744; 4: 0.756; 5: 0.618; 1: 75; 2: 0.520; 3: 0.546; 4: 0.533; 5: 0.395; 1: 37.5; 2: 0.304; 3: 0.324; 4: 0.314; 5: 0.176; 1: 18.75; 2: 0.248; 3: 0.241; 4: 0.245; 5: 0.107; 1: 0; 2: 0.136; 3: 0.140; 4: 0.138 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Rat SOST ELISA Kit was designed for the quantitative measurement of Rat SOST protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.68 pg/mL. |
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