Fish Insulin-like growth factor II(IGF2) ELISA kit CSB-EL011088FI CUSABIO
$50.00
In stock
SKU
CSB-EL011088FI
Product Details
| Target Name | insulin-like growth factor 2 (somatomedin A) |
| Uniprot No. | Q02816 |
| Species | Fish |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 31.25 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | igf2Insulin-like growth factor II ELISA Kit; IGF-II ELISA Kit; Erythrotropin ELISA Kit |
| Abbreviation | IGF2 |
| Sample Types | serum, plasma |
| Detection Range | 62.5 pg/mL-1000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Metabolism |
| Assay Principle | quantitative |
| Measurement | Competitive |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:100; 2: Average %; 3: 91; 1: Range %; 2: 87-102; 1: 1:200; 2: Average %; 3: 95; 1: Range %; 2: 91-99; 1: 1:400; 2: Average %; 3: 96; 1: Range %; 2: 88-106; 1: 1:800; 2: Average %; 3: 92; 1: Range %; 2: 88-96 |
| Recovery | headers: The recovery of fish IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of fish IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of fish IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 96; 3: 90-102; 1: EDTA plasma (n=4); 2: 93; 3: 85-102; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml OD1 OD2 Average 1000 0.085 0.089 0.087 500 0.192 0.205 0.199 250 0.485 0.489 0.487 125 0.942 0.937 0.940 62.5 1.613 1.572 1.593 0 2.595 2.624 2.610; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 9: 1000; 10: 0.085; 11: 0.089; 12: 0.087; 16: 500; 17: 0.192; 18: 0.205; 19: 0.199; 23: 250; 24: 0.485; 25: 0.489; 26: 0.487; 30: 125; 31: 0.942; 32: 0.937; 33: 0.940; 37: 62.5; 38: 1.613; 39: 1.572; 40: 1.593; 44: 0; 45: 2.595; 46: 2.624; 47: 2.610; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 1: 1000; 2: 0.085; 3: 0.089; 4: 0.087; 1: 500; 2: 0.192; 3: 0.205; 4: 0.199; 1: 250; 2: 0.485; 3: 0.489; 4: 0.487; 1: 125; 2: 0.942; 3: 0.937; 4: 0.940; 1: 62.5; 2: 1.613; 3: 1.572; 4: 1.593; 1: 0; 2: 2.595; 3: 2.624; 4: 2.610 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Fish IGF2 ELISA Kit was designed for the quantitative measurement of Fish IGF2 protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 62.5 pg/mL-1000 pg/mL and the sensitivity is 31.25 pg/mL. |
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