Rat hypoxia-inducible factor 1α,HIF-1α ELISA Kit CSB-E08540r CUSABIO
$50.00
In stock
SKU
CSB-E08540r
Product Details
| Target Name | hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) |
| Uniprot No. | O35800 |
| Species | Rattus norvegicus (Rat) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.78 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Hif1aHypoxia-inducible factor 1-alpha ELISA Kit; HIF-1-alpha ELISA Kit; HIF1-alpha ELISA Kit |
| Abbreviation | HIF1A |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 3.12 pg/mL-200 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Epigenetics and Nuclear Signaling |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of rat HIF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of rat HIF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of rat HIF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 90; 1: Range %; 2: 87-94; 1: 1:2; 2: Average %; 3: 96; 1: Range %; 2: 91-100; 1: 1:4; 2: Average %; 3: 93; 1: Range %; 2: 87-97; 1: 1:8; 2: Average %; 3: 100; 1: Range %; 2: 96-103 |
| Recovery | headers: The recovery of rat HIF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of rat HIF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of rat HIF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 92; 3: 87-95; 1: EDTA plasma (n=4); 2: 95; 3: 88-98; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 200 2.715 2.656 2.686 2.538 100 1.730 1.676 1.703 1.555 50 1.196 1.179 1.188 1.040 25 0.608 0.618 0.613 0.465 12.5 0.404 0.412 0.408 0.260 6.25 0.303 0.316 0.310 0.162 3.12 0.248 0.237 0.243 0.095 0 0.150 0.146 0.148; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 200; 9: 2.715; 10: 2.656; 11: 2.686; 12: 2.538; 14: 100; 15: 1.730; 16: 1.676; 17: 1.703; 18: 1.555; 20: 50; 21: 1.196; 22: 1.179; 23: 1.188; 24: 1.040; 26: 25; 27: 0.608; 28: 0.618; 29: 0.613; 30: 0.465; 32: 12.5; 33: 0.404; 34: 0.412; 35: 0.408; 36: 0.260; 38: 6.25; 39: 0.303; 40: 0.316; 41: 0.310; 42: 0.162; 44: 3.12; 45: 0.248; 46: 0.237; 47: 0.243; 48: 0.095; 50: 0; 51: 0.150; 52: 0.146; 53: 0.148; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 200; 2: 2.715; 3: 2.656; 4: 2.686; 5: 2.538; 1: 100; 2: 1.730; 3: 1.676; 4: 1.703; 5: 1.555; 1: 50; 2: 1.196; 3: 1.179; 4: 1.188; 5: 1.040; 1: 25; 2: 0.608; 3: 0.618; 4: 0.613; 5: 0.465; 1: 12.5; 2: 0.404; 3: 0.412; 4: 0.408; 5: 0.260; 1: 6.25; 2: 0.303; 3: 0.316; 4: 0.310; 5: 0.162; 1: 3.12; 2: 0.248; 3: 0.237; 4: 0.243; 5: 0.095; 1: 0; 2: 0.150; 3: 0.146; 4: 0.148 |
| Materials provided | A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Rat HIF-1α antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled HIF-1α antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody. One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution. One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Rat HIF1A ELISA Kit was designed for the quantitative measurement of Rat HIF1A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL . |
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