Rat macrophage inflammatory protein 1α,MIP-1α ELISA Kit CSB-E07422r CUSABIO
$50.00
In stock
SKU
CSB-E07422r
Product Details
| Target Name | chemokine (C-C motif) ligand 3 |
| Uniprot No. | P50229 |
| Species | Rattus norvegicus (Rat) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3.12 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Ccl3 ELISA Kit; Mip1a ELISA Kit; Scya3 ELISA Kit; C-C motif chemokine 3 ELISA Kit; Macrophage inflammatory protein 1-alpha ELISA Kit; MIP-1-alpha ELISA Kit; Small-inducible cytokine A3 ELISA Kit |
| Abbreviation | CCL3 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 12.5 pg/mL-800 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 88; 1: Range %; 2: 84-98; 1: 1:2; 2: Average %; 3: 96; 1: Range %; 2: 91-104; 1: 1:4; 2: Average %; 3: 99; 1: Range %; 2: 96-105; 1: 1:8; 2: Average %; 3: 95; 1: Range %; 2: 89-99 |
| Recovery | headers: The recovery of rat MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of rat MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of rat MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 104; 3: 99-108; 1: EDTA plasma (n=4); 2: 95; 3: 91-104; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 800 2.152 2.051 2.102 1.969 400 1.484 1.383 1.434 1.301 200 0.888 0.867 0.878 0.745 100 0.522 0.511 0.517 0.384 50 0.336 0.347 0.342 0.209 25 0.253 0.264 0.259 0.126 12.5 0.195 0.199 0.197 0.064 0 0.132 0.133 0.133; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 800; 9: 2.152; 10: 2.051; 11: 2.102; 12: 1.969; 14: 400; 15: 1.484; 16: 1.383; 17: 1.434; 18: 1.301; 20: 200; 21: 0.888; 22: 0.867; 23: 0.878; 24: 0.745; 26: 100; 27: 0.522; 28: 0.511; 29: 0.517; 30: 0.384; 32: 50; 33: 0.336; 34: 0.347; 35: 0.342; 36: 0.209; 38: 25; 39: 0.253; 40: 0.264; 41: 0.259; 42: 0.126; 44: 12.5; 45: 0.195; 46: 0.199; 47: 0.197; 48: 0.064; 50: 0; 51: 0.132; 52: 0.133; 53: 0.133; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 800; 2: 2.152; 3: 2.051; 4: 2.102; 5: 1.969; 1: 400; 2: 1.484; 3: 1.383; 4: 1.434; 5: 1.301; 1: 200; 2: 0.888; 3: 0.867; 4: 0.878; 5: 0.745; 1: 100; 2: 0.522; 3: 0.511; 4: 0.517; 5: 0.384; 1: 50; 2: 0.336; 3: 0.347; 4: 0.342; 5: 0.209; 1: 25; 2: 0.253; 3: 0.264; 4: 0.259; 5: 0.126; 1: 12.5; 2: 0.195; 3: 0.199; 4: 0.197; 5: 0.064; 1: 0; 2: 0.132; 3: 0.133; 4: 0.133 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Chemokine (C-C motif) ligand 3 (CCL3), also known as macrophage inflammatory protein 1α (MIP-1α), is a small cytokine belonging to the CC chemokine family. This protein recruits and activates immune cells, including macrophages, T cells, and natural killer cells, to sites of inflammation or infection. Multiple cell types produce CCL3, which acts as a chemoattractant that guides immune cell migration and activation during both innate and adaptive immune responses. CCL3 serves as an important mediator in inflammatory diseases and immune system regulation. The Rat macrophage inflammatory protein 1α,MIP-1α ELISA Kit (CSB-E07422r) is designed for quantitative measurement of CCL3 in Rattus norvegicus samples. This sandwich ELISA works with serum, plasma, and tissue homogenates with a detection range of 12.5 pg/mL-800 pg/mL and sensitivity of 3.12 pg/mL. The assay requires 50-100ul sample volume, operates with detection at 450 nm wavelength, and can be completed within 1-5 hours. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. Researchers have used this ELISA kit to study inflammatory responses and cytokine profiling in various experimental contexts. Studies have applied the kit to quantify MIP-1α levels alongside other inflammatory mediators to examine immune system activation and inflammatory cascades. The kit has proven valuable for measuring this chemokine in both systemic circulation and localized tissue environments. • Inflammatory research: Quantification of MIP-1α as part of comprehensive cytokine panels to evaluate inflammatory responses and immune system activation • Arthritis and joint disease studies: Measurement of MIP-1α levels in both serum and synovial fluid to examine local and systemic inflammatory status in joint-related pathology • Biomarker profiling: Integration with multi-analyte approaches to characterize inflammatory mediator patterns alongside other cytokines and growth factors • Comparative inflammatory analysis: Measurement of MIP-1α levels in conjunction with related chemokines and cytokines to understand broader inflammatory networks |
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