Rat matrix metalloproteinase 1(MMP-1/Collagenase I) ELISA kit CSB-E07416r CUSABIO
$50.00
In stock
SKU
CSB-E07416r
Product Details
| Uniprot No. | P81563 |
| Species | Rattus norvegicus (Rat) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.058 ng/ml |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Mmp1Interstitial collagenase ELISA Kit; EC 3.4.24.7 ELISA Kit; Fibroblast collagenase ELISA Kit; Matrix metalloproteinase-1 ELISA Kit; MMP-1 ELISA Kit; Myocardial collagenase ELISA Kit; Fragment ELISA Kit |
| Abbreviation | MMP1 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.235 ng/ml - 15 ng/ml |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cancer |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-1/Collagenase I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-1/Collagenase I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-1/Collagenase I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 89; 1: Range %; 2: 85-94; 1: 1:2; 2: Average %; 3: 92; 1: Range %; 2: 87-98; 1: 1:4; 2: Average %; 3: 95; 1: Range %; 2: 90-102; 1: 1:8; 2: Average %; 3: 100; 1: Range %; 2: 96-109 |
| Recovery | headers: The recovery of rat MMP-1/Collagenase I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of rat MMP-1/Collagenase I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of rat MMP-1/Collagenase I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 84; 3: 80-89; 1: EDTA plasma (n=4); 2: 97; 3: 90-104 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 15; 2: 2.257; 3: 2.231; 4: 2.244; 5: 2.147; 1: 7.5; 2: 1.876; 3: 1.832; 4: 1.854; 5: 1.757; 1: 3.75; 2: 1.381; 3: 1.373; 4: 1.377; 5: 1.280; 1: 1.88; 2: 0.835; 3: 0.797; 4: 0.816; 5: 0.719; 1: 0.94; 2: 0.397; 3: 0.376; 4: 0.387; 5: 0.290; 1: 0.47; 2: 0.273; 3: 0.257; 4: 0.265; 5: 0.168; 1: 0.235; 2: 0.197; 3: 0.207; 4: 0.202; 5: 0.105; 1: 0; 2: 0.099; 3: 0.094; 4: 0.097 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Matrix metalloproteinase-1 (MMP-1), also known as interstitial collagenase, is a zinc-dependent endopeptidase that functions in extracellular matrix remodeling. This enzyme specifically cleaves fibrillar collagens, particularly types I, II, and III. It is necessary for tissue development, wound healing, and various pathological processes. Fibroblasts, keratinocytes, and other cell types produce MMP-1 in response to inflammatory stimuli and growth factors. Dysregulated MMP-1 activity has been linked to numerous diseases including arthritis, cancer metastasis, and cardiovascular disorders, making it an important biomarker for research applications. This quantitative sandwich ELISA kit detects rat matrix metalloproteinase-1 in serum, plasma, and tissue homogenates. The assay offers a detection range of 0.235 ng/ml to 15 ng/ml with a sensitivity of 0.058 ng/ml. The protocol requires 50-100 μl sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, providing researchers with a reliable tool for measuring MMP-1 levels in Rattus norvegicus samples. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been used in research examining matrix degradation processes and inflammatory responses in experimental animal models. The kit allows quantification of matrix metalloproteinase-1 in serum samples from rats subjected to monoiodoacetate-induced joint pathology models. • Matrix degradation research: Analysis of collagenase activity in experimental arthritis models to understand cartilage and extracellular matrix breakdown processes • Inflammatory biomarker profiling: Measurement of matrix metalloproteinase levels alongside pro-inflammatory cytokines and pain-related factors in serum samples • Joint pathology studies: Examination of matrix remodeling enzymes in monoiodoacetate-induced osteoarthritis models to evaluate disease progression and tissue degradation • Multi-analyte biomarker panels: Integration with cytokine and growth factor measurements to provide comprehensive analysis of inflammatory and tissue remodeling responses |
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