Fish Interleukin-8,IL-8 ELISA Kit CSB-E12877Fh CUSABIO
$50.00
In stock
SKU
CSB-E12877Fh
Product Details
| Species | Fish |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 31.25 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Abbreviation | IL8/CXCL8 |
| Sample Types | serum, plasma |
| Detection Range | 31.25 pg/mL-500 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Competitive |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<15%; rows: Intra-assay Precision (Precision within an assay): CV%<15%: Intra-assay Precision (Precision within an assay): CV%<15%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<15%: Inter-assay Precision (Precision between assays): CV%<15%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 93; 1: Range %; 2: 92-97; 1: 1:2; 2: Average %; 3: 95; 1: Range %; 2: 85-100; 1: 1:4; 2: Average %; 3: 93; 1: Range %; 2: 89-101; 1: 1:8; 2: Average %; 3: 95; 1: Range %; 2: 86-100 |
| Recovery | headers: The recovery of fish IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of fish IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of fish IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 96; 3: 89-100; 1: EDTA plasma (n=4); 2: 95; 3: 85-105; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml OD1 OD2 Average 500 0.172 0.176 0.174 250 0.392 0.399 0.396 125 0.635 0.628 0.632 62.5 1.038 1.046 1.042 31.25 2.456 2.417 2.437; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 9: 500; 10: 0.172; 11: 0.176; 12: 0.174; 16: 250; 17: 0.392; 18: 0.399; 19: 0.396; 23: 125; 24: 0.635; 25: 0.628; 26: 0.632; 30: 62.5; 31: 1.038; 32: 1.046; 33: 1.042; 37: 31.25; 38: 2.456; 39: 2.417; 40: 2.437; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 1: 500; 2: 0.172; 3: 0.176; 4: 0.174; 1: 250; 2: 0.392; 3: 0.399; 4: 0.396; 1: 125; 2: 0.635; 3: 0.628; 4: 0.632; 1: 62.5; 2: 1.038; 3: 1.046; 4: 1.042; 1: 31.25; 2: 2.456; 3: 2.417; 4: 2.437 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Fish IL8/CXCL8 ELISA Kit was designed for the quantitative measurement of Fish IL8/CXCL8 protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 31.25 pg/mL-500 pg/mL and the sensitivity is 31.25 pg/mL. |
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