Rat sex hormone-binding globulin (SHBG) ELISA kit CSB-E12118r CUSABIO
$50.00
In stock
SKU
CSB-E12118r
Product Details
| Target Name | sex hormone-binding globulin |
| Uniprot No. | P08689 |
| Species | Rattus norvegicus (Rat) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 375 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Shbg ELISA Kit; Sex hormone-binding globulin ELISA Kit; SHBG ELISA Kit; Sex steroid-binding protein ELISA Kit; SBP ELISA Kit; Testis-specific androgen-binding protein ELISA Kit; ABP ELISA Kit |
| Abbreviation | SHBG |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates, cell lysates |
| Detection Range | 375 ng/mL-6000 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Competitive |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<15%; rows: Intra-assay Precision (Precision within an assay): CV%<15%: Intra-assay Precision (Precision within an assay): CV%<15%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<15%: Inter-assay Precision (Precision between assays): CV%<15%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of rat SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of rat SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of rat SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 92; 1: Range %; 2: 88-96; 1: 1:2; 2: Average %; 3: 88; 1: Range %; 2: 85-90; 1: 1:4; 2: Average %; 3: 100; 1: Range %; 2: 97-103; 1: 1:8; 2: Average %; 3: 83; 1: Range %; 2: 80-86 |
| Recovery | headers: The recovery of rat SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of rat SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of rat SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 87; 3: 82-91; 1: EDTA plasma (n=4); 2: 95; 3: 89-100; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average 6000 0.204 0.201 0.203 3000 0.336 0.341 0.339 1500 0.789 0.772 0.781 750 1.800 1.806 1.803 375 2.312 2.353 2.333; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 8: 6000; 9: 0.204; 10: 0.201; 11: 0.203; 14: 3000; 15: 0.336; 16: 0.341; 17: 0.339; 20: 1500; 21: 0.789; 22: 0.772; 23: 0.781; 26: 750; 27: 1.800; 28: 1.806; 29: 1.803; 32: 375; 33: 2.312; 34: 2.353; 35: 2.333; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 1: 6000; 2: 0.204; 3: 0.201; 4: 0.203; 1: 3000; 2: 0.336; 3: 0.341; 4: 0.339; 1: 1500; 2: 0.789; 3: 0.772; 4: 0.781; 1: 750; 2: 1.800; 3: 1.806; 4: 1.803; 1: 375; 2: 2.312; 3: 2.353; 4: 2.333 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Sex hormone-binding globulin (SHBG) is a glycoprotein produced primarily in the liver that serves as the major transport protein for sex hormones including testosterone, dihydrotestosterone, and estradiol in blood circulation. SHBG regulates the bioavailability of these steroid hormones by binding them with high affinity, thereby controlling the amount of free, biologically active hormone available to target tissues. Changes in SHBG levels can significantly impact hormone homeostasis and are associated with various physiological and pathological conditions. This makes SHBG an important biomarker in endocrine research and reproductive biology studies. The Rat sex hormone-binding globulin (SHBG) ELISA kit (CSB-E12118r) provides quantitative measurement of SHBG in Rattus norvegicus samples. This competitive assay works with multiple sample types including serum, plasma, cell culture supernates, tissue homogenates, and cell lysates. The kit offers a detection range of 375 ng/mL to 6000 ng/mL with a sensitivity of 375 ng/mL. The assay requires 50-100 μL sample volume, operates with detection at 450 nm wavelength, and can be completed within 1-5 hours. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been used in endocrine research to quantify sex hormone-binding globulin levels in rat serum samples. Researchers have applied the assay as part of comprehensive hormonal profiling studies examining multiple sex hormones and their binding proteins in laboratory animal models. • Endocrine profiling: Used alongside other hormone assays to measure sex hormone-binding globulin concentrations as part of broader hormonal panel evaluations in rat studies • Serum biomarker analysis: Applied for quantitative measurement of sex hormone-binding globulin in rat serum samples to study hormonal regulation and binding protein dynamics • Reproductive endocrinology research: Used in studies examining sex hormone metabolism and transport protein levels in experimental animal models |
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
