Fish metallothionein(MT)ELISA kit CSB-EQ027262FI CUSABIO
$50.00
In stock
SKU
CSB-EQ027262FI
Product Details
| Target Name | metallothionein(MT) |
| Species | Fish |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 12.5 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Abbreviation | MT |
| Sample Types | serum, plasma |
| Detection Range | 25 pg/mL-400 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Epigenetics and Nuclear Signaling |
| Assay Principle | quantitative |
| Measurement | Competitive |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of fish MT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of fish MT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of fish MT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:100; 2: Average %; 3: 84; 1: Range %; 2: 80-88; 1: 1:200; 2: Average %; 3: 90; 1: Range %; 2: 86-94; 1: 1:400; 2: Average %; 3: 99; 1: Range %; 2: 94-103; 1: 1:800; 2: Average %; 3: 92; 1: Range %; 2: 88-96 |
| Recovery | headers: The recovery of fish MT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of fish MT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of fish MT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 97; 3: 92-102; 1: EDTA plasma (n=4); 2: 95; 3: 89-99; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average 400 0.103 0.100 0.102 200 0.247 0.228 0.238 100 0.401 0.387 0.394 50 0.646 0.598 0.622 25 0.983 0.966 0.975 0 1.572 1.529 1.551; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 8: 400; 9: 0.103; 10: 0.100; 11: 0.102; 14: 200; 15: 0.247; 16: 0.228; 17: 0.238; 20: 100; 21: 0.401; 22: 0.387; 23: 0.394; 26: 50; 27: 0.646; 28: 0.598; 29: 0.622; 32: 25; 33: 0.983; 34: 0.966; 35: 0.975; 38: 0; 39: 1.572; 40: 1.529; 41: 1.551; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 1: 400; 2: 0.103; 3: 0.100; 4: 0.102; 1: 200; 2: 0.247; 3: 0.228; 4: 0.238; 1: 100; 2: 0.401; 3: 0.387; 4: 0.394; 1: 50; 2: 0.646; 3: 0.598; 4: 0.622; 1: 25; 2: 0.983; 3: 0.966; 4: 0.975; 1: 0; 2: 1.572; 3: 1.529; 4: 1.551 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Fish metallothionein (MT) ELISA Kit was designed for the quantitative measurement of Fish metallothionein (MT) protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 25 pg/mL-400 pg/mL and the sensitivity is 12.5 pg/mL. |
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