Goat Leptin,LEP ELISA kit CSB-E12834G CUSABIO
$50.00
In stock
SKU
CSB-E12834G
Product Details
| Target Name | leptin |
| Uniprot No. | Q257X2 |
| Species | Capra hircus (Goat) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.156 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | LEP ELISA Kit; OBLeptin ELISA Kit; Obesity factor ELISA Kit |
| Abbreviation | LEP |
| Sample Types | serum, plasma |
| Detection Range | 0.625 ng/mL-40 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Metabolism |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of goat LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of goat LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of goat LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 89; 1: Range %; 2: 82-97; 1: 1:2; 2: Average %; 3: 91; 1: Range %; 2: 85-99; 1: 1:4; 2: Average %; 3: 92; 1: Range %; 2: 86-100; 1: 1:8; 2: Average %; 3: 94; 1: Range %; 2: 84-98 |
| Recovery | headers: The recovery of goat LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of goat LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of goat LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 96; 3: 90-99; 1: EDTA plasma (n=4); 2: 93; 3: 85-101; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 40 1.960 1.934 1.947 1.805 20 1.742 1.795 1.769 1.627 10 1.425 1.397 1.411 1.269 5 0.983 1.003 0.993 0.851 2.5 0.655 0.649 0.652 0.510 1.25 0.431 0.424 0.428 0.286 0.625 0.248 0.239 0.244 0.102 0 0.141 0.143 0.142; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 40; 9: 1.960; 10: 1.934; 11: 1.947; 12: 1.805; 14: 20; 15: 1.742; 16: 1.795; 17: 1.769; 18: 1.627; 20: 10; 21: 1.425; 22: 1.397; 23: 1.411; 24: 1.269; 26: 5; 27: 0.983; 28: 1.003; 29: 0.993; 30: 0.851; 32: 2.5; 33: 0.655; 34: 0.649; 35: 0.652; 36: 0.510; 38: 1.25; 39: 0.431; 40: 0.424; 41: 0.428; 42: 0.286; 44: 0.625; 45: 0.248; 46: 0.239; 47: 0.244; 48: 0.102; 50: 0; 51: 0.141; 52: 0.143; 53: 0.142; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 40; 2: 1.960; 3: 1.934; 4: 1.947; 5: 1.805; 1: 20; 2: 1.742; 3: 1.795; 4: 1.769; 5: 1.627; 1: 10; 2: 1.425; 3: 1.397; 4: 1.411; 5: 1.269; 1: 5; 2: 0.983; 3: 1.003; 4: 0.993; 5: 0.851; 1: 2.5; 2: 0.655; 3: 0.649; 4: 0.652; 5: 0.510; 1: 1.25; 2: 0.431; 3: 0.424; 4: 0.428; 5: 0.286; 1: 0.625; 2: 0.248; 3: 0.239; 4: 0.244; 5: 0.102; 1: 0; 2: 0.141; 3: 0.143; 4: 0.142 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Goat LEP ELISA Kit was designed for the quantitative measurement of Goat LEP protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL. |
MDSD
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