Recombinant Dog B-lymphocyte antigen CD20 (MS4A1)-VLPs (Active) CSB-MP661636DO CUSABIO
$558.00
In stock
SKU
CSB-MP661636DO
Product Details
| Uniprot No. | Q3C2E2 |
| Alternative Names | (Membrane-spanning 4-domains subfamily A member 1)(CD antigen CD20) |
| Species | Canis lupus familiaris (Dog) (Canis familiaris) |
| Source | Mammalian cell |
| Expression Region | 1-297aa |
| Purity | Greater than 90% as determined by SEC-HPLC. |
| Endotoxin | Less than 1.0 EU/ug as determined by LAL method. |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized Dog CD20 at 10 μg/mL can bind Anti-CD20 recombinant antibody (CSB-RA015007MA3HU), the EC 50 is 147.3-199.0 ng/mL. |
| Tag Info | C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions) |
| Form | Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4 |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs | Protein FAQs |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Lead Time | Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
| Target Names | MS4A1 |
| Target Protein Sequence | MTTPRNSMSGTLPVDPMKSPTAMYPVQKIIPKRMPSVVGPTQNFFMRESKTLGAVQIMNGLFHIALGSLLMIHTDVCAPICITMWYPLWGGIMFIISGSLLAAADKNPRKSLVKGKMIMNSLSLFAAISGIIFLIMDIFNITISHFFKMENLNLIKAPMPYVDIHNCDPANPSEKNSLSIQYCGSIRSVFLGVFAVMLIFAFFQKLVTAGIVENEWKKLCSKPKSDVVVLLAAEEKKEQPIETTEEMVELTEIASQPKKEEDIEIIPVQEEEGELEINFAEPPQEQESSPIENDSIP |
| Mol. Weight | 34.4 kDa |
| Protein Length | Full Length |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Datasheet & COA | Please contact us to get it. |
| Description | CD20 presents a significant challenge as a multi-pass transmembrane target, and this virus-like particle (VLP) format preserves the native conformation of full-length canine CD20 (aa 1–297) by displaying it within a membrane context—critical for generating conformationally relevant immune responses and accurate binding data. Functional ELISA confirms that immobilized dog CD20-VLPs bind an anti-CD20 recombinant antibody with an EC50 of 147.3–199.0 ng/mL, validating the protein's suitability as an antigen for therapeutic antibody screening, cross-reactivity assessment, and immunization campaigns targeting canine lymphoma. The mammalian expression system ensures proper folding and post-translational modifications, while endotoxin levels below 1.0 EU/μg satisfy criteria typical for cell-based functional assays and in vivo immunization studies in veterinary oncology models. The C-terminal 10×His tag, detectable under denaturing conditions, supports use in Western blot-based quality control without interfering with native-conformation binding assays. |
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