Human 14-3-3 protein beta/alpha (YWHAB) ELISA kit CSB-E12010h CUSABIO
$50.00
In stock
SKU
CSB-E12010h
Product Details
| Target Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide |
| Uniprot No. | P31946 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 1.56 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | 14 3 3 ELISA Kit; 14 3 3 protein beta ELISA Kit; 14 3 3 protein beta/alpha ELISA Kit; 14 3 3 protein zeta ELISA Kit; 14 3 3 zeta ELISA Kit; 14-3-3 protein beta/alpha ELISA Kit; 14-3-3 protein/cytosolic phospholipase A2 ELISA Kit; 1433B_HUMAN ELISA Kit; GW128 ELISA Kit; HS1 ELISA Kit; KCIP 1 ELISA Kit; KCIP-1 ELISA Kit; MGC111427 ELISA Kit; MGC126532 ELISA Kit; MGC138156 ELISA Kit; N-terminally processed ELISA Kit; Protein 1054 ELISA Kit; Protein kinase C inhibitor protein 1 ELISA Kit; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; delta polypeptide ELISA Kit; Tyrosine 3/tryptophan 5 -monooxygenase activation protein; zeta polypeptide ELISA Kit; YWHAB ELISA Kit; YWHAD ELISA Kit; YWHAZ ELISA Kit |
| Abbreviation | YWHAB |
| Sample Types | serum, plasma, tissue homogenates, cell lysates |
| Detection Range | 6.25 ng/mL-400 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Others |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human YWHAB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human YWHAB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human YWHAB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 92; 1: Range %; 2: 87-96; 1: 1:2; 2: Average %; 3: 94; 1: Range %; 2: 90-98; 1: 1:4; 2: Average %; 3: 89; 1: Range %; 2: 84-93; 1: 1:8; 2: Average %; 3: 84; 1: Range %; 2: 80-88 |
| Recovery | headers: The recovery of human YWHAB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human YWHAB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human YWHAB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 92; 3: 88-95; 1: EDTA plasma (n=4); 2: 97; 3: 91-103; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 400 2.552 2.602 2.577 2.456 200 1.749 1.797 1.773 1.652 100 1.028 1.083 1.056 0.935 50 0.569 0.543 0.556 0.435 25 0.338 0.316 0.327 0.206 12.5 0.246 0.240 0.243 0.122 6.25 0.167 0.161 0.164 0.043 0 0.125 0.116 0.121; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 400; 9: 2.552; 10: 2.602; 11: 2.577; 12: 2.456; 14: 200; 15: 1.749; 16: 1.797; 17: 1.773; 18: 1.652; 20: 100; 21: 1.028; 22: 1.083; 23: 1.056; 24: 0.935; 26: 50; 27: 0.569; 28: 0.543; 29: 0.556; 30: 0.435; 32: 25; 33: 0.338; 34: 0.316; 35: 0.327; 36: 0.206; 38: 12.5; 39: 0.246; 40: 0.240; 41: 0.243; 42: 0.122; 44: 6.25; 45: 0.167; 46: 0.161; 47: 0.164; 48: 0.043; 50: 0; 51: 0.125; 52: 0.116; 53: 0.121; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 400; 2: 2.552; 3: 2.602; 4: 2.577; 5: 2.456; 1: 200; 2: 1.749; 3: 1.797; 4: 1.773; 5: 1.652; 1: 100; 2: 1.028; 3: 1.083; 4: 1.056; 5: 0.935; 1: 50; 2: 0.569; 3: 0.543; 4: 0.556; 5: 0.435; 1: 25; 2: 0.338; 3: 0.316; 4: 0.327; 5: 0.206; 1: 12.5; 2: 0.246; 3: 0.240; 4: 0.243; 5: 0.122; 1: 6.25; 2: 0.167; 3: 0.161; 4: 0.164; 5: 0.043; 1: 0; 2: 0.125; 3: 0.116; 4: 0.121 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human YWHAB ELISA Kit was designed for the quantitative measurement of Human YWHAB protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL . |
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