Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2(SRD5A2) ELISA kit CSB-EL022654HU CUSABIO
$50.00
In stock
SKU
CSB-EL022654HU
Product Details
| Target Name | steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) |
| Uniprot No. | P31213 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 6 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | SRD5A2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; 5 alpha-SR2; SR type 2; Steroid 5-alpha-reductase 2; S5AR 2; Type II 5-alpha reductase |
| Abbreviation | SRD5A2 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 12 pg/mL-2400 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Competitive |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<15%; rows: Intra-assay Precision (Precision within an assay): CV%<15%: Intra-assay Precision (Precision within an assay): CV%<15%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<15%: Inter-assay Precision (Precision between assays): CV%<15%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human SRD5A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human SRD5A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human SRD5A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 88; 1: Range %; 2: 85-93; 1: 1:2; 2: Average %; 3: 91; 1: Range %; 2: 87-98; 1: 1:4; 2: Average %; 3: 94; 1: Range %; 2: 89-99; 1: 1:8; 2: Average %; 3: 94; 1: Range %; 2: 82-97 |
| Recovery | headers: The recovery of human SRD5A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human SRD5A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human SRD5A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 96; 3: 90-102; 1: EDTA plasma (n=4); 2: 97; 3: 91-104; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average 0 2.053 2.137 2.095 12 1.498 1.488 1.493 48 1.086 1.052 1.069 192 0.591 0.620 0.606 600 0.261 0.276 0.269 2400 0.114 0.115 0.115; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 8: 0; 9: 2.053; 10: 2.137; 11: 2.095; 14: 12; 15: 1.498; 16: 1.488; 17: 1.493; 20: 48; 21: 1.086; 22: 1.052; 23: 1.069; 26: 192; 27: 0.591; 28: 0.620; 29: 0.606; 32: 600; 33: 0.261; 34: 0.276; 35: 0.269; 38: 2400; 39: 0.114; 40: 0.115; 41: 0.115; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 1: 0; 2: 2.053; 3: 2.137; 4: 2.095; 1: 12; 2: 1.498; 3: 1.488; 4: 1.493; 1: 48; 2: 1.086; 3: 1.052; 4: 1.069; 1: 192; 2: 0.591; 3: 0.620; 4: 0.606; 1: 600; 2: 0.261; 3: 0.276; 4: 0.269; 1: 2400; 2: 0.114; 3: 0.115; 4: 0.115 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human SRD5A2 ELISA Kit was designed for the quantitative measurement of Human SRD5A2 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 12 pg/mL-2400 pg/mL and the sensitivity is 6 pg/mL. |
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