Human A Disintegrin And Metalloprotease 33,ADAM33 ELISA Kit CSB-E12702h CUSABIO
$50.00
In stock
SKU
CSB-E12702h
Product Details
| Target Name | ADAM metallopeptidase domain 33 |
| Uniprot No. | Q9BZ11 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 7.8 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | A disintegrin and metalloprotease 33 ELISA Kit; A disintegrin and metalloproteinase domain 33 ELISA Kit; ADA33_HUMAN ELISA Kit; ADAM 33 ELISA Kit; ADAM metallopeptidase domain 33 ELISA Kit; ADAM33 ELISA Kit; C20orf153 ELISA Kit; Disintegrin and metalloproteinase domain-containing protein 33 ELISA Kit; Disintegrin and reprolysin metalloproteinase family protein ELISA Kit; DJ964F7.1 ELISA Kit; DKFZp434K0521 ELISA Kit; FLJ35308 ELISA Kit; FLJ36751 ELISA Kit; Metalloprotease disintegrin ELISA Kit; MGC149823 ELISA Kit; MGC71889 ELISA Kit; PRO1891 ELISA Kit; UNQ873 ELISA Kit |
| Abbreviation | ADAM33 |
| Sample Types | serum, cell culture supernates, saliva, tissue homogenates |
| Detection Range | 31.25 pg/mL-2000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cell Biology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 97; 1: Range %; 2: 92-101; 1: 1:2; 2: Average %; 3: 89; 1: Range %; 2: 83-93; 1: 1:4; 2: Average %; 3: 92; 1: Range %; 2: 88-96; 1: 1:8; 2: Average %; 3: 95; 1: Range %; 2: 90-100 |
| Recovery | headers: The recovery of human ADAM33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human ADAM33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human ADAM33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 93; 3: 89-97; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 2000 2.748 2.672 2.710 2.581 1000 2.131 2.005 2.068 1.939 500 1.491 1.486 1.489 1.360 250 0.966 0.935 0.951 0.822 125 0.546 0.574 0.560 0.431 62.5 0.363 0.392 0.378 0.249 31.25 0.254 0.243 0.249 0.120 0 0.125 0.132 0.129; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 2000; 9: 2.748; 10: 2.672; 11: 2.710; 12: 2.581; 14: 1000; 15: 2.131; 16: 2.005; 17: 2.068; 18: 1.939; 20: 500; 21: 1.491; 22: 1.486; 23: 1.489; 24: 1.360; 26: 250; 27: 0.966; 28: 0.935; 29: 0.951; 30: 0.822; 32: 125; 33: 0.546; 34: 0.574; 35: 0.560; 36: 0.431; 38: 62.5; 39: 0.363; 40: 0.392; 41: 0.378; 42: 0.249; 44: 31.25; 45: 0.254; 46: 0.243; 47: 0.249; 48: 0.120; 50: 0; 51: 0.125; 52: 0.132; 53: 0.129; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 2000; 2: 2.748; 3: 2.672; 4: 2.710; 5: 2.581; 1: 1000; 2: 2.131; 3: 2.005; 4: 2.068; 5: 1.939; 1: 500; 2: 1.491; 3: 1.486; 4: 1.489; 5: 1.360; 1: 250; 2: 0.966; 3: 0.935; 4: 0.951; 5: 0.822; 1: 125; 2: 0.546; 3: 0.574; 4: 0.560; 5: 0.431; 1: 62.5; 2: 0.363; 3: 0.392; 4: 0.378; 5: 0.249; 1: 31.25; 2: 0.254; 3: 0.243; 4: 0.249; 5: 0.120; 1: 0; 2: 0.125; 3: 0.132; 4: 0.129 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human ADAM33 ELISA Kit was designed for the quantitative measurement of Human ADAM33 protein in serum, cell culture supernates, saliva, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL. |
MDSD