Human Afamin(AFM) ELISA kit CSB-EL001419HU CUSABIO
$50.00
In stock
SKU
CSB-EL001419HU
Product Details
| Target Name | afamin |
| Uniprot No. | P43652 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.97 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | AFAM_HUMAN ELISA Kit; Afamin ELISA Kit; Afm ELISA Kit; ALB 2 ELISA Kit; ALB2 ELISA Kit; ALBA ELISA Kit; ALF ELISA Kit; Alpha albumin ELISA Kit; Alpha-Alb ELISA Kit; Alpha-albumin ELISA Kit |
| Abbreviation | AFM |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 3.9 ng/mL-250 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human AFM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human AFM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human AFM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1000; 2: Average %; 3: 96; 1: Range %; 2: 90-102; 1: 1:2000; 2: Average %; 3: 89; 1: Range %; 2: 84-95; 1: 1:4000; 2: Average %; 3: 100; 1: Range %; 2: 95-106; 1: 1:8000; 2: Average %; 3: 97; 1: Range %; 2: 92-104 |
| Recovery | headers: The recovery of human AFM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human AFM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human AFM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 89; 3: 93-96; 1: EDTA plasma (n=4); 2: 102; 3: 98-107; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 250 2.024 2.098 2.061 1.929 125 1.715 1.761 1.738 1.606 62.5 1.334 1.364 1.349 1.217 31.25 0.893 0.884 0.889 0.757 15.6 0.567 0.546 0.557 0.425 7.8 0.321 0.349 0.335 0.203 3.9 0.261 0.246 0.254 0.122 0 0.131 0.133 0.132; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 250; 9: 2.024; 10: 2.098; 11: 2.061; 12: 1.929; 14: 125; 15: 1.715; 16: 1.761; 17: 1.738; 18: 1.606; 20: 62.5; 21: 1.334; 22: 1.364; 23: 1.349; 24: 1.217; 26: 31.25; 27: 0.893; 28: 0.884; 29: 0.889; 30: 0.757; 32: 15.6; 33: 0.567; 34: 0.546; 35: 0.557; 36: 0.425; 38: 7.8; 39: 0.321; 40: 0.349; 41: 0.335; 42: 0.203; 44: 3.9; 45: 0.261; 46: 0.246; 47: 0.254; 48: 0.122; 50: 0; 51: 0.131; 52: 0.133; 53: 0.132; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 250; 2: 2.024; 3: 2.098; 4: 2.061; 5: 1.929; 1: 125; 2: 1.715; 3: 1.761; 4: 1.738; 5: 1.606; 1: 62.5; 2: 1.334; 3: 1.364; 4: 1.349; 5: 1.217; 1: 31.25; 2: 0.893; 3: 0.884; 4: 0.889; 5: 0.757; 1: 15.6; 2: 0.567; 3: 0.546; 4: 0.557; 5: 0.425; 1: 7.8; 2: 0.321; 3: 0.349; 4: 0.335; 5: 0.203; 1: 3.9; 2: 0.261; 3: 0.246; 4: 0.254; 5: 0.122; 1: 0; 2: 0.131; 3: 0.133; 4: 0.132 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | The product CSB-EL001419HU is a sandwich ELISA kit developed to measure levels of human Afamin (AFM) in multiple samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of AFM bound in the initial step. AFM is a human plasma vitamin E-binding glycoprotein mainly produced in the liver. It promotes vitamin E transport across the blood-brain barrier. AFM is also a tumor marker for ovarian cancer. AFM has been found strongly associated with all components of metabolic syndrome including visceral obesity and has been a potential early marker of metabolic syndrome. It also plays a role in anti-apoptotic cellular processes associated with oxidative stress and is linked to insulin resistance and other features of metabolic syndrome. |
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