Human Apoptosis-associated speck-like protein containing a CARD(PYCARD) ELISA kit CSB-EL019114HU CUSABIO
$50.00
In stock
SKU
CSB-EL019114HU
Product Details
| Target Name | PYD and CARD domain containing |
| Uniprot No. | Q9ULZ3 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3.9 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Apoptosis associated speck like protein containing a CARD ELISA Kit; Apoptosis-associated speck-like protein containing a CARD ELISA Kit; ASC ELISA Kit; ASC_HUMAN ELISA Kit; CARD 5 ELISA Kit; CARD5 ELISA Kit; Caspase recruitment domain containing protein 5 ELISA Kit; Caspase recruitment domain protein 5 ELISA Kit; Caspase recruitment domain-containing protein 5 ELISA Kit; hASC ELISA Kit; MGC10332 ELISA Kit; PYCARD ELISA Kit; PYD and CARD domain containing ELISA Kit; PYD and CARD domain containing protein ELISA Kit; PYD and CARD domain-containing protein ELISA Kit; Target of methylation induced silencing 1 ELISA Kit; Target of methylation-induced silencing 1 ELISA Kit; TMS 1 ELISA Kit; TMS ELISA Kit; TMS1 ELISA Kit |
| Abbreviation | PYCARD |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 15.6 pg/mL-1000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cell Biology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human PYCARD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human PYCARD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human PYCARD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 95; 1: Range %; 2: 90-102; 1: 1:2; 2: Average %; 3: 90; 1: Range %; 2: 85-99; 1: 1:4; 2: Average %; 3: 98; 1: Range %; 2: 92-106; 1: 1:8; 2: Average %; 3: 92; 1: Range %; 2: 86-100 |
| Recovery | headers: The recovery of human PYCARD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human PYCARD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human PYCARD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 94; 3: 89-98; 1: EDTA plasma (n=4); 2: 95; 3: 90-100; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 1000 1.834 1.867 1.851 1.713 500 1.517 1.541 1.529 1.391 250 1.213 1.224 1.219 1.081 125 0.892 0.902 0.897 0.759 62.5 0.561 0.578 0.570 0.432 31.2 0.345 0.352 0.349 0.211 15.6 0.256 0.266 0.261 0.123 0 0.137 0.139 0.138; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 1000; 9: 1.834; 10: 1.867; 11: 1.851; 12: 1.713; 14: 500; 15: 1.517; 16: 1.541; 17: 1.529; 18: 1.391; 20: 250; 21: 1.213; 22: 1.224; 23: 1.219; 24: 1.081; 26: 125; 27: 0.892; 28: 0.902; 29: 0.897; 30: 0.759; 32: 62.5; 33: 0.561; 34: 0.578; 35: 0.570; 36: 0.432; 38: 31.2; 39: 0.345; 40: 0.352; 41: 0.349; 42: 0.211; 44: 15.6; 45: 0.256; 46: 0.266; 47: 0.261; 48: 0.123; 50: 0; 51: 0.137; 52: 0.139; 53: 0.138; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 1000; 2: 1.834; 3: 1.867; 4: 1.851; 5: 1.713; 1: 500; 2: 1.517; 3: 1.541; 4: 1.529; 5: 1.391; 1: 250; 2: 1.213; 3: 1.224; 4: 1.219; 5: 1.081; 1: 125; 2: 0.892; 3: 0.902; 4: 0.897; 5: 0.759; 1: 62.5; 2: 0.561; 3: 0.578; 4: 0.570; 5: 0.432; 1: 31.2; 2: 0.345; 3: 0.352; 4: 0.349; 5: 0.211; 1: 15.6; 2: 0.256; 3: 0.266; 4: 0.261; 5: 0.123; 1: 0; 2: 0.137; 3: 0.139; 4: 0.138 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | PYCARD, also known as apoptosis-associated speck-like protein containing a CARD, is an adaptor protein that plays a central role in innate immune signaling and inflammasome assembly. This protein contains both a pyrin domain (PYD) and a caspase activation and recruitment domain (CARD), allowing it to bridge interactions between pattern recognition receptors and downstream effector proteins. PYCARD is essential for the formation of inflammasomes, multi-protein complexes that activate inflammatory caspases and process and secrete pro-inflammatory cytokines such as IL-1β and IL-18, making it a key mediator in inflammatory responses and cell death pathways. This Human PYCARD ELISA kit (CSB-EL019114HU) uses a quantitative sandwich measurement principle to detect PYD and CARD domain containing protein in human samples. The assay works with serum, plasma, and tissue homogenates with a detection range of 15.6 pg/mL to 1000 pg/mL and shows a sensitivity of 3.9 pg/mL. The protocol requires 50-100 μL sample volume and can be completed within 1-5 hours, with detection performed at 450 nm wavelength. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been used in respiratory disease research to quantify ASC protein levels in lung tissue homogenates. The kit supports studies of inflammasome pathway components and their role in inflammatory responses. • Respiratory disease research: Measuring ASC protein expression in lung tissue to understand inflammasome activation patterns • Inflammasome pathway studies: Quantifying ASC alongside other inflammasome components including NLRP3 and caspase-1 to evaluate inflammatory signaling cascades • Cytokine network analysis: Integration with inflammatory mediator profiling to examine relationships between inflammasome components and pro-inflammatory cytokine production • Tissue-based protein analysis: Application in homogenized tissue samples to measure target protein levels in disease-relevant biological specimens |
MDSD
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
