CDC25B Recombinant Monoclonal Antibody CSB-RA004995A0HU CUSABIO
$210.00
In stock
SKU
CSB-RA004995A0HU
Product Details
| Uniprot No. | P30305 |
| Alternative Names | AI604853 antibody; Cdc 25B antibody; Cdc25b antibody; CDC25HU2 antibody; Cdc25m2 antibody; Cell division cycle 25 homolog B antibody; Cell division cycle 25B antibody; Cell division cycle 25B isoform 1 antibody; Cell division cycle 25B isoform 2 antibody; Cell division cycle 25B isoform 3 antibody; Cell division cycle 25B isoform 4 antibody; Cell division cycle 25B isoform 5 antibody; Dual specificity phosphatase Cdc25B antibody; EC 3.1.3.48 antibody; M phase inducer phosphatase 2 antibody; M-phase inducer phosphatase 2 antibody; MPIP2_HUMAN antibody |
| Species Reactivity | Human |
| Immunogen Species | Homo sapiens (Human) |
| Immunogen | A synthesized peptide derived from human CDC25B |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Purification Method | Affinity-chromatography |
| Concentration | It differs from different batches. Please contact us to confirm it. |
| Form | Liquid |
| Buffer | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Tested Applications | ELISA |
| Troubleshooting and FAQs | Antibody FAQs |
| Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
| Usage | For Research Use Only. Not for use in diagnostic or therapeutic procedures. |
| Target Names | CDC25B |
| Conjugate | Non-conjugated |
| Clone No. | 3D6 |
| Protocols | ELISA Protocol |
| Description | The CDC25B recombinant monoclonal antibody is produced using DNA recombinant technology and in vitro genetic manipulation. It involves immunizing animals with a synthesized peptide derived from human CDC25B to isolate B cells and select positive B cells. These positive B cells undergo screening and identification of individual clones. The light and heavy chains of the CDC25B antibody are then amplified using PCR and inserted into a plasmid vector. This recombinant vector is transfected into a host cell line for antibody expression. The CDC25B recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. It specifically binds to human CDC25B protein and is recommended for ELISA. CDC25B is a phosphatase enzyme that plays a critical role in the regulation of the cell cycle. Its main function is to activate the CDK complexes that drive the progression of the cell cycle through the G2/M checkpoint. This activation occurs by dephosphorylating specific residues on the CDK complexes, which allows them to bind to and phosphorylate their target substrates, leading to mitosis and cell division. CDC25B also plays a role in DNA damage response and repair. It can be activated in response to DNA damage, leading to increased cell cycle arrest and DNA repair mechanisms. |
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