CDC45 Recombinant Monoclonal Antibody CSB-RA005020A0HU CUSABIO
$210.00
In stock
SKU
CSB-RA005020A0HU
Product Details
| Uniprot No. | O75419 |
| Alternative Names | CDC 45 antibody; CDC 45L antibody; CDC45 (cell division cycle 45; S.cerevisiae homolog) like antibody; CDC45 antibody; CDC45 cell division cycle 45 like antibody; CDC45 like antibody; CDC45 related protein antibody; CDC45_HUMAN antibody; CDC45L2 antibody; Cell division control protein 45 homolog antibody; Cell division cycle 45 like 2 antibody; Cell division cycle 45 like antibody; PORC PI 1 antibody; PORC-PI-1 antibody |
| Species Reactivity | Human |
| Immunogen Species | Homo sapiens (Human) |
| Immunogen | A synthesized peptide derived from human CDC45 |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Purification Method | Affinity-chromatography |
| Concentration | It differs from different batches. Please contact us to confirm it. |
| Form | Liquid |
| Buffer | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Tested Applications | ELISA |
| Troubleshooting and FAQs | Antibody FAQs |
| Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
| Usage | For Research Use Only. Not for use in diagnostic or therapeutic procedures. |
| Target Names | CDC45 |
| Conjugate | Non-conjugated |
| Clone No. | 4B4 |
| Protocols | ELISA Protocol |
| Description | The CDC45 recombinant monoclonal antibody is generated using DNA recombinant technology and in vitro genetic manipulation. The process begins with the immunization of an animal using a synthesized peptide derived from human CDC45, followed by the isolation of B cells and subsequent selection of positive B cells. These selected B cells undergo screening and identification of single clones. The light and heavy chains of the CDC45 antibody are amplified through PCR and inserted into a plasmid vector to create a recombinant vector. This vector is then introduced into a host cell line for antibody expression. The CDC45 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. It specifically recognizes human CDC45 protein and is particularly useful in ELISA. The CDC45 is a member of the conserved pre-initiation complex (pre-IC) that forms at the origin of replication and helps to initiate DNA replication. Specifically, CDC45 binds to other proteins in the pre-IC, such as MCM2-7 and GINS, to form the active CMG helicase complex, which unwinds the double-stranded DNA at the origin of replication, allowing other replication machinery to access the DNA and begin the process of copying the genetic material. CDC45 has also been implicated in other cellular processes, such as the DNA damage response and cell cycle regulation. |
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